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  4. Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2)

Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2)

  • 中文名称：
    Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2)
  • 货号：
    CSB-EP518991DOA(A4)
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    HAP2
  • Uniprot No.：
    F4JP36
  • 别名：
    GENERATIVE CELL SPECIFIC 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    82.3 kDa
  • 表达区域：
    25-705aa
  • 氨基酸序列
    IQILSKSKLEKCEKTSDSGNLNCSTKIVLNLAVPSGSSGGEASIVAEIVEVEDNSSSNMQTVRIPPVITVNKSAAYALYDLTYIRDVPYKPQEYHVTTRKCEHDAGPDIVQICERLRDEKGNVLEQTQPICCPCGPQRRMPSSCGDIFDKMIKGKANTAHCLRFPGDWFHVFGIGQRSLGFSVRVELKTGTRVSEVIIGPENRTATANDNFLKVNLIGDFGGYTSIPSFEDFYLVIPREAAEAGQPGSLGANYSMWMLLERVRFTLDGLECNKIGVGYEAFNTQPNFCSSPYWSCLHNQLWNFRESDINRIDRHQLPLYGLEGRFERINQHPNAGPHSFSIGVTETLNTNLMIELRADDIEYVFQRSPGKIINIAIPTFEALTQFGVAAVIIKNTGEVEASYSLTFDCSKGVAFVEEQFFIIKPKAVTTRSFKLYPTKDQAAKYICTAILKDSQFSEVDRAECQFSTTATVLDNGTQVTNPFQIPETQPKGFFDSIRILWTKIINGLVDFITGDTCRNKCSSFFDFSCHIQYVCLSWMVMFGLLLALFPITCLLLWLLHQKGLFDPCYDWWEDHFDLDHHRRLLPSRADVVNRHHHHHKHRHHHNHHRRTHQRHKHHHGQDDDVLQKMMLERDHSDSHYYHQLHRVHKDSKQKQRRRAKHGIVLPRDVHVERQRKQRLRES
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-Myc-tagged and C-terminal Flag-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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HAP2 Proteins

靶点详情

  • 功能：
    Required for male fertility. Plays a role in pollen tube guidance and successful gamete attachment. Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm. Mediates the fusion of cell membranes. Not required for pollen tube outgrowth.
  • 基因功能参考文献：
    1. no significant difference in ovule-targeting frequency between hap2-1 and HAP2 pollen tubes, indicating that the gcs1/hap2 mutation does not affect the ability of pollen tubes to target the ovule; results supply a clue that may allow conclusive settlement of the disagreement regarding the function of GCS1/HAP2 during the angiosperm sexual reproduction process PMID: 28791484
    2. These results provide data identifying the essential functional features of HAP2, a highly conserved sperm fusion protein. PMID: 20333238
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    HAP2/GCS1 family
  • 组织特异性：
    Expressed only in mature pollen, in the two sperm cells.
  • 数据库链接：

    KEGG: ath:AT4G11720

    STRING: 3702.AT4G11720.1

    UniGene: At.54282

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