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  4. Recombinant Arabidopsis thaliana Protein EMBRYONIC FLOWER 1 (EMF1), partial

Recombinant Arabidopsis thaliana Protein EMBRYONIC FLOWER 1 (EMF1), partial

  • 中文名称：
    拟南芥EMF1重组蛋白
  • 货号：
    CSB-YP878636DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥EMF1重组蛋白
  • 货号：
    CSB-EP878636DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥EMF1重组蛋白
  • 货号：
    CSB-EP878636DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥EMF1重组蛋白
  • 货号：
    CSB-BP878636DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥EMF1重组蛋白
  • 货号：
    CSB-MP878636DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EMF1
  • Uniprot No.：
    Q9LYD9
  • 别名：
    EMF1; At5g11530; F15N18.120Protein EMBRYONIC FLOWER 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription repressor that regulates phase transition during shoot, flower and seeds development. Controls leaves development, shoot architecture and flowering by delaying both the vegetative to reproductive transition and flower initiation. Participates in polycomb group (PcG) protein complex-mediated (including EMF2) silencing of the flower homeotic genes AGAMOUS (AG), PISTILLATA (PI), and APETALA3 (AP3), as well as of some regulatory genes such as ABSCISIC ACID INSENSITIVE3 (ABI3), LONG VEGETATIVE PHASE1 (LOV1), and FLOWERING LOCUS C (FLC) during vegetative development. Required for histone methylation or for maintaining a stable histone methylation (e.g. H3K27me3) pattern of repressed target genes (including genes involved in salt stress response and flower development); this repression is counteracted by ULT1. Can bind non specifically DNA (both double- and single-stranded) and RNA.
  • 基因功能参考文献：
    1. to promote vegetative development and to regulate cell differentiation during shoot and root organogenesis, EMF1 binds genes required for other developmental phases. These are primarily genes trimethylated by EMF2-PRC2 on their H3K27 PMID: 22457632
    2. EMF1 interacts with EIP1, EIP6 and EIP9 during vegetative development to regulate flowering time in Arabidopsis. PMID: 21700722
    3. EMF1 and EMF2 target transcription factor genes to mediate vegetative development and stress responses, and regulate flowering time genes. PMID: 19783648
    4. Results are consistent with EMF1's primary role in repressing flowering in order to allow for vegetative growth. PMID: 19825645
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in mature embryo, root tips, cotyledons, leaves, stems, shoot apex, and flower clusters, with highest levels in flowers. The presence in the shoot apical meristem (SAM) is required to maintain vegetative development and prevent early flowering.
  • 数据库链接：

    KEGG: ath:AT5G11530

    STRING: 3702.AT5G11530.1

    UniGene: At.43396

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