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  4. Recombinant Arabidopsis thaliana Probable peptide/nitrate transporter At3g47960 (At3g47960), partial

Recombinant Arabidopsis thaliana Probable peptide/nitrate transporter At3g47960 (At3g47960), partial

  • 中文名称：
    拟南芥NPF2.10重组蛋白
  • 货号：
    CSB-YP821983DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥NPF2.10重组蛋白
  • 货号：
    CSB-EP821983DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥NPF2.10重组蛋白
  • 货号：
    CSB-EP821983DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥NPF2.10重组蛋白
  • 货号：
    CSB-BP821983DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥NPF2.10重组蛋白
  • 货号：
    CSB-MP821983DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NPF2.10
  • Uniprot No.：
    Q944G5
  • 别名：
    NPF2.10; GTR1; At3g47960; T17F15.170; Protein NRT1/ PTR FAMILY 2.10; AtNPF2.10; Protein GLUCOSINOLATE TRANSPORTER-1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    High-affinity, proton-dependent glucosinolate-specific transporter. Involved in the distribution of glucosinolates within the leaf, including import into the glucosinolate-rich S-cells located adjacent to the phloem. Involved in bidirectional long-distance transport of aliphatic but not indole glucosinolates. May be involved in removal of glucosinolates from the xylem in roots.
  • 基因功能参考文献：
    1. Expression of glucosinolate (GLS) importers GTR1 and GTR2 inside the stele, along with earlier observations, suggest three possible routes of stele-synthesized aliphatic GLS after their synthesis: (i) GTR-dependent import to cells symplastically connected to cortical cells and rhizosphere; (ii) GTR-independent transport via xylem to the shoot; and (iii) GTR-dependent import to GLS-degrading myrosin cells at the cortex. PMID: 27702989
    2. dimerization of GTR1, possibly induced by dephosphorylation of the Thr residue located between the possible transmembrane regions, regulates its plasma membrane localization. PMID: 28594299
    3. GTR1 would be involved in the translocation of JA and JA-Ile in plant and may be contributed to correct positioning of JA and JA-Ile to attenuate an excessive wound response in undamaged leaves PMID: 27760496
    4. Phosphorylation of AtGTR1 is not directly involved in regulating AtGTR1 transport activity. PMID: 26340317
    5. GTR1 role of transport processes in leaf glucosinolate distribution PMID: 25209984
    6. glucosinolate can specifically translocate from rosette and roots to inflorescence in a GTR1- and GTR2-dependent manner PMID: 24481282
    7. GTR1 and GTR2 control the loading of glucosinolates from the apoplasm into the phloem. [GTR1] PMID: 22864417

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    PTR2/POT transporter (TC 2.A.17) family
  • 组织特异性：
    Expressed in stems, flowers, siliques, roots, shoots and leaves. Expressed in veins and in adjacent mesophyll cells in leaves, and in the root vasculature with highest expression in lateral branching points.
  • 数据库链接：

    KEGG: ath:AT3G47960

    STRING: 3702.AT3G47960.1

    UniGene: At.20263

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