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  4. Recombinant Arabidopsis thaliana Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY (SPY), partial

Recombinant Arabidopsis thaliana Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY (SPY), partial

  • 中文名称：
    拟南芥SPY重组蛋白
  • 货号：
    CSB-YP846546DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥SPY重组蛋白
  • 货号：
    CSB-EP846546DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥SPY重组蛋白
  • 货号：
    CSB-EP846546DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥SPY重组蛋白
  • 货号：
    CSB-BP846546DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥SPY重组蛋白
  • 货号：
    CSB-MP846546DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPY
  • Uniprot No.：
    Q96301
  • 别名：
    SPY; At3g11540; F24K9.29; Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY; EC 2.4.1.255
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Functions with GIGANTEA (GI) in pathways controlling flowering, circadian cotyledon movements and hypocotyl elongation. Acts as a light-regulated promoter of elongation via its interaction with GI. Acts as an activator of cytokinin signaling. Required with SEC for gamete and seed development. Its OGT activity has been proved in vitro but not in vivo.
  • 基因功能参考文献：
    1. posttranslational modification of TCP14/15 by SPY inhibits their proteolysis. PMID: 27208284
    2. The study revealed that SPY-dependent protein O-fucosylation plays a key role in regulating plant development. PMID: 28244988
    3. SPINDLY regulates cortex proliferation by maintaining cellular redox homeostasis PMID: 25267734
    4. SPY plays a negative role in plant abiotic stress tolerance, probably by integrating environmental stress signals via gibberellic acid and Cytokinins cross talk. PMID: 22013217
    5. Data suggest that responses of leaves and flowers to CK are mediated by SPY-dependent TCP14 and TCP15 activities. PMID: 22267487
    6. spy-4 partially suppressed the reduced abundance of CONSTANS and FLOWERING LOCUS T RNA and the late flowering of gigantea mutant plants PMID: 15155885
    7. Our results demonstrate that SPY acts as both a repressor of GA responses and a positive regulator of cytokinin signaling. Hence, SPY may play a central role in the regulation of GA/cytokinin cross talk during plant development. PMID: 15608330
    8. SPY acts in the cytosol to suppress gibberellin responses and to promote cytokinin signaling. PMID: 19228341

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Glycosyltransferase 41 family, O-GlcNAc transferase subfamily
  • 组织特异性：
    Widely expressed. Present throughout the plant (at protein level).
  • 数据库链接：

    KEGG: ath:AT3G11540

    STRING: 3702.AT3G11540.1

    UniGene: At.17656

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