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  4. Recombinant Arabidopsis thaliana Presequence protease 1, chloroplastic/mitochondrial (PREP1), partial

Recombinant Arabidopsis thaliana Presequence protease 1, chloroplastic/mitochondrial (PREP1), partial

  • 中文名称：
    拟南芥PREP1重组蛋白
  • 货号：
    CSB-YP864777DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PREP1重组蛋白
  • 货号：
    CSB-EP864777DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PREP1重组蛋白
  • 货号：
    CSB-EP864777DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PREP1重组蛋白
  • 货号：
    CSB-BP864777DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PREP1重组蛋白
  • 货号：
    CSB-MP864777DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PREP1
  • Uniprot No.：
    Q9LJL3
  • 别名：
    PREP1; ZNMP1; At3g19170; MVI11.6; MVI11.7; MVI11_8Presequence protease 1; chloroplastic/mitochondrial; AtPreP1; PreP 1; EC 3.4.24.-; Zinc metalloprotease 1; AtZnMP1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, with a bias for positively charged amino acid residues.
  • 基因功能参考文献：
    1. Data show that the AtPreP presequence has the capacity to target GFP into the mitochondrial matrix of all three species. PMID: 21621546
    2. AtPreP functions as a peptidasome that degrades targeting peptides and other small unstructured peptides. In addition to Zn located in the catalytic site, AtPreP also contains two Mg-binding sites. [PreP1] PMID: 19646442
    3. AtPreP1 and AtPreP2 genes are differentially expressed in Arabidopsis PMID: 17081117
    4. The AtPreP peptidasome is required for efficient plant growth and organelle function particularly during early development. PMID: 19701724
    5. AtPrePI and AtPrePII are metalloproteases that degrade targeting peptides in mitochondria and chloroplasts. AtPrePI showed a preference for the N-terminal amphiphilic α-helix and positively charged amino acid residues. PMID: 15827031

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  • 亚细胞定位：
    Plastid, chloroplast stroma. Mitochondrion matrix.
  • 蛋白家族：
    Peptidase M16 family, PreP subfamily
  • 组织特异性：
    Expressed only in siliques and flowers.
  • 数据库链接：

    KEGG: ath:AT3G19170

    STRING: 3702.AT3G19170.1

    UniGene: At.27915

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