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  4. Recombinant Arabidopsis thaliana Potassium channel SKOR (SKOR), partial

Recombinant Arabidopsis thaliana Potassium channel SKOR (SKOR), partial

  • 中文名称：
    拟南芥SKOR重组蛋白
  • 货号：
    CSB-YP864960DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥SKOR重组蛋白
  • 货号：
    CSB-EP864960DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥SKOR重组蛋白
  • 货号：
    CSB-EP864960DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥SKOR重组蛋白
  • 货号：
    CSB-BP864960DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥SKOR重组蛋白
  • 货号：
    CSB-MP864960DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SKOR
  • Uniprot No.：
    Q9M8S6
  • 别名：
    SKOR; At3g02850; F13E7.21; Potassium channel SKOR; Stelar K(+) outward rectifying channel
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Highly selective outward-rectifying potassium channel. Involved in potassium release into the xylem sap toward the shoots. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by depolarization. The voltage-dependence of the channel is abolished by internal or external acidification. May interact with the cytoskeleton or with regulatory proteins.
  • 基因功能参考文献：
    1. Data show that nutritional conditions as a critical factor for the joint activity of Arabidopsis proteins SKOR and NPF7.3/NRT1.5 for shoot potassium (K) homeostasis. PMID: 26508776
    2. Study found that, in particular, the N-terminal part of the S5 played a critical role in KAT1 and SKOR gating. PMID: 20007672
    3. A minimal cysteine motif required to activate the SKOR K+ channel of Arabidopsis by the reactive oxygen species H2O2. PMID: 20605786
    4. Phosphatidylinositol phosphates were identified as essential regulators for the voltage-dependent and -independent activation of A. thaliana potassium channels expressed in X. laevis oocytes. PMID: 15842627
    5. The intracellular potassium sensing mechanism couples SKOR activity to potassium nutrition status in the 'source cells', establishing a supply-based unloading system for the regulation of potassium distribution. PMID: 16623888
    6. Mapping the SKOR sequence to the crystal structure of the voltage-dependent K+ channel suggests interaction between the S6 gating domain and the base of the pore helix, a prediction supported by mutations at this site. PMID: 16623889
    7. Single mutations in SKOR convert it from an outward K+ channel into an inward K+ channel. PMID: 18287042

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Potassium channel family, Plant (TC 1.A.1.4) subfamily
  • 组织特异性：
    Expressed in root pericycle and xylem parenchyma, and in flower at a lower level.
  • 数据库链接：

    KEGG: ath:AT3G02850

    STRING: 3702.AT3G02850.1

    UniGene: At.41071

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