Recombinant Arabidopsis thaliana Phototropin-1 (PHOT1), partial

1. Data show that phototropin 1 (phot1), which senses low light to promote positive phototropism (growth towards the light), also limits the response in high light. PMID: 29921904
2. the kinase domain of Arabidopsis phot1 and phot2 can be successfully engineered to accommodate non-natural ATP analogues by substituting the bulky gatekeeper residue threonine for glycine. PMID: 29475950
3. The spatial targeting approach therefore excludes the hypocotyl apex as the site for light perception for phototropism and shows that phot1-mediated NPH3 de-phosphorylation is tissue autonomous and occurs more prominently in the basal hypocotyl. PMID: 27545835
4. Data suggest that Arabidopsis can adapt to elevated phosphorylation status of phototropin-1 R472H mutant in part by reducing its stability, whereas activity of the mutant under high-light conditions can be attributed to additional increases in LOV2-mediated photoreceptor autophosphorylation. [phot1] PMID: 28663371
5. Data suggest that Arabidopsis can adapt to elevated phosphorylation status of phototropin-1 R472H mutant in part by reducing its stability, whereas activity of the mutant under high-light conditions can be attributed to additional increases in LOV2-mediated photoreceptor autophosphorylation. PMID: 28663371
6. lysine 475, a functionally important residue, in the N-terminal region of LOV2 plays a critical role in transmitting the structural changes in LOV2 to the kinase domain. The interface between the domains is critical for signaling, suitably changing the structure to activate the kinase in response to conformational changes in the adjoining LOV2. PMID: 27484797
7. phot1 and phot2 phosphorylate BLUE LIGHT SIGNALING1 (BLUS1) kinase as a common substrate in stomatal opening. phot1 phosphorylates BLUS1 more efficiently. The phosphorylation status of BLUS1 is a key determinant of physiological response. PMID: 26780063
8. After cold treatment, in darkness, the expression of phototropin1 is down-regulated, while phototropin2 levels are up-regulated. The motile system of chloroplasts in Arabidopsis is more sensitive to blue light at low temperature, similar to other species. PMID: 26398808
9. PHOT1 plays a role(s) in elongation of lateral roots through the control of an auxin-related signalling pathway. PMID: 24803136
10. Truncation of the A'alpha-helix and AA substitutions at Glu474 and Lys475 in the gap between the A'alpha and the Abeta strand of LOV2 to Ala impaired the BL-induced activation of the STK, but did not affect S390 formation. PMID: 25886203
11. The phot1-mediated adjustment of phytochrome-regulated photomorphogenic events is most robust in dim blue light conditions and is likely modulated by auxin transport through its transporters. PMID: 25482790
12. A. thaliana phototropin 1 LOV2 domain is a dimer in which two alpha helices adopt a scissor-like orientation at the dimer interface and form a short alpha-helical coiled coil. PMID: 24316821
13. phototropic bending of etiolated Arabidopsis seedlings requires phot1 in the upper hypocotyl, below the cotyledonary node, to perceive the phototropic stimulus and induce a signaling cascade that rapidly spreads to all cell layers in the hypocotyl. PMID: 24076239
14. Phot1 mediates the high-intensity blue light-induced cytosolic calcium increases in hypocotyls. PMID: 23674105
15. RPT2 function in the phot1 signaling pathway. PMID: 22739508
16. PHOT1 functions in mediating chloroplast accumulation. PHOT1 LOV2 domain is essential to maintain developing primary leaves in a horizontal position under white light from above. PMID: 22782888
17. phototropin 1 localizes on the chloroplast outer membrane PMID: 23161859
18. Phot1 and Phot2 promote leaf flattening by suppressing the leaf-curling activity of phyB. PMID: 23054390
19. the photosensitivity of kinase activation in phot involves the lifetime of S390 in LOV2, suggesting that the lifetime is one of the key factors for the different photosensitivities observed for phot1 and phot2 PMID: 23066024
20. Phytochrome Kinase Substrate 4 (PKS4), a known element of phot1 signalling, is a substrate of phot1 kinase activity in vitro and is phosphorylated in a phot1-dependent manner in vivo. PMID: 22781128
21. The plasma membrane-associated phototropic receptor phot1 is ubiquitinated in response to blue light activation. Ubiquitination of phot1 is dependent upon both the phot1-interacting proteins NPH3 and CUL3. PMID: 21990941
22. Leaf epidermal peels of Arabidopsis mutants lacking phototropins 1 and 2 exposed to a background of red light show severely impaired stomatal opening responses to blue light. PMID: 22147516
23. photoregulation of the kinase in phototropin1 (phot1) PMID: 22001205
24. No reorganization of chloropast-actin filaments was seen in phot2 or phot1phot2 mutants lacking phot2, and jac1 mutants, lacking the accumulation response, showed no avoidance movements under the whole-cell irradiation condition. PMID: 21737483
25. The fluence-response kinetics and time course were similar to the phototropin1 mediated response observed following a blue pulse. PMID: 20861684
26. Findings indicate that while phot1-5/phot2-1 plants also responded to changes in light intensity, the resulting pattern of chloroplast distribution was quite different from that in WT. PMID: 20614182
27. phot1 is a blue-light receptor kinases controlling a range of responses that optimize the photosynthetic efficiency of Arabidosis. PMID: 20031924
28. Coexpression of active and inactive forms of phot1 demonstrates that autophosphorylation can occur intermolecularly, independent of LOV1, via light-dependent receptor dimerization in vivo. PMID: 19880798
29. We conclude that phototropins promote plant growth by controlling and integrating a variety of responses that optimize photosynthetic performance under low photosynthetically active radiation in the natural environment. PMID: 15749755
30. The interaction of JAC1 with PHOT1 and PHOT2 in chloroplast movement in A. thaliana is reported. PMID: 16113208
31. LOV2 domain acts as a major light-regulated molecular switch of casein phosphorylation, & it acts as an attenuator of the light activation of the kinase by LOV2. LOV1 domain acts as an attenuator of the light activation of the kinase by LOV2. PMID: 16150710
32. PKS1 interacts with PHOT1. PMID: 16777956
33. The light, oxygen, or voltage domain (LOV2) of phot1 plays a major role in phototropism and leaf expansion. PMID: 17085510
34. Phototropin-1 enhances performance under drought by mediating plastic increases in root growth efficiency near the soil surface. PMID: 17176396
35. A two-step mechanism of the linker unfolding is proposed. PMID: 17275025
36. exert a positive effect on phototropic curvature under long-term irradiation conditions. Specifically, in a phot1-deficient genetic background (phot1 mutant), curvature is reduced in the absence of cryptochromes. PMID: 18183416
37. Data suggest that 5PTase13 antagonizes PHOT1-mediated effects on calcium signaling under blue light. PMID: 18252844
38. autophosphorylation of Ser-851 is a primary step that mediates signaling between photochemical reaction and physiological events. PMID: 18378899
39. LOV1 domain of Phot1 and Phot2 forms a dimer through face-to-face association of beta-scaffolds in the crystallographic asymmetric unit. PMID: 18585389
40. The recombinant LOV1 domains of phototropin 1 and phototropin 2 have been crystallized; the crystal of the LOV1 domain of phototropin 1 belongs to orthorhombic space group P2(1)2(1)2(1) and diffracts X-rays to a resolution of 2.1 angstroms. PMID: 18607090
41. Phot1 is more sensitive to light stimuli than phot2. The N-terminal moiety of phot1 mainly determines the sensitivity of the response. PMID: 18643969
42. Data show that the dissociation of the linker region from the LOV2 domain is a key step for the conformation change of the phot1LOV2-linker to induce biological activity, and suggest that the phototropins could have a role as a temperature sensors. PMID: 18793649
43. Localization of phot1 in Arabidopsis is mediated by phytochrome A. PMID: 18952772
44. phot1-LOV2 is in a monomer-dimer equilibrium and phot2-LOV2 exists as a monomer in the dark PMID: 19166850
45. Phototropism of inflorescence stems and leaf petioles is also controlled by phototropins. phot1 works as a major photoreceptor under low and high light conditions. PMID: 19689999

显示更多

收起更多

KEGG: ath:AT3G45780

STRING: 3702.AT3G45780.1

UniGene: At.3720