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  4. Recombinant Arabidopsis thaliana Phosphomethylpyrimidine synthase, chloroplastic (THIC), partial

Recombinant Arabidopsis thaliana Phosphomethylpyrimidine synthase, chloroplastic (THIC), partial

  • 中文名称：
    拟南芥THIC重组蛋白
  • 货号：
    CSB-YP526870DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥THIC重组蛋白
  • 货号：
    CSB-EP526870DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥THIC重组蛋白
  • 货号：
    CSB-EP526870DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥THIC重组蛋白
  • 货号：
    CSB-BP526870DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥THIC重组蛋白
  • 货号：
    CSB-MP526870DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    THIC
  • Uniprot No.：
    O82392
  • 别名：
    THIC; PY; At2g29630; T27A16.27; Phosphomethylpyrimidine synthase; chloroplastic; EC 4.1.99.17; Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Protein PYRIMIDINE REQUIRING; Thiamine biosynthesis protein ThiC; Protein THIAMINE C
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.
  • 基因功能参考文献：
    1. the expression of TenA_E and ThiC genes in Arabidopsis and maize was inversely correlated. PMID: 25014715
    2. Thic protein crystal structure shows a metal ion with multiple coordinated water molecules in the close vicinity of the substrate binding sites. PMID: 24161603
    3. In Arabidopsis, the THIC promoter and the thiamin-pyrophosphate riboswitch act simultaneously to tightly regulate thiamin biosynthesis in a circadian manner and consequently sense and control vital points of core cellular metabolism. PMID: 23341335
    4. The results suggest that vitamin B1 biosynthesis in plants is in fact more similar to prokaryotic counterparts and that the THIC protein is likely to be the key regulatory protein in the pathway. PMID: 18048325
  • 亚细胞定位：
    Plastid, chloroplast stroma.
  • 蛋白家族：
    ThiC family
  • 组织特异性：
    Strongly expressed in cotyledons, leaves, flowers and siliques, and, to a lower extent, in roots. Expressed in all green tissues, but not in roots, seeds, and petals.
  • 数据库链接：

    KEGG: ath:AT2G29630

    STRING: 3702.AT2G29630.1

    UniGene: At.24797

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