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  4. Recombinant Arabidopsis thaliana Pectinesterase/pectinesterase inhibitor 3 (PME3), partial

Recombinant Arabidopsis thaliana Pectinesterase/pectinesterase inhibitor 3 (PME3), partial

  • 中文名称：
    拟南芥PME3重组蛋白
  • 货号：
    CSB-YP527400DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PME3重组蛋白
  • 货号：
    CSB-EP527400DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PME3重组蛋白
  • 货号：
    CSB-EP527400DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PME3重组蛋白
  • 货号：
    CSB-BP527400DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PME3重组蛋白
  • 货号：
    CSB-MP527400DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PME3
  • Uniprot No.：
    O49006
  • 别名：
    PME3; ARATH27; At3g14310; MLN21.10; Pectinesterase/pectinesterase inhibitor 3 [Includes: Pectinesterase inhibitor 3; Pectin methylesterase inhibitor 3); Pectinesterase 3; PE 3; EC 3.1.1.11; Pectin methylesterase 27; AtPME27; Pectin methylesterase 3)]
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts in the modification of cell walls via demethylesterification of cell wall pectin.
  • 基因功能参考文献：
    1. alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings PMID: 28375469
    2. Docking analysis indicated that the inhibition of AtPME3 could occur via the interaction of AtPMEI7 with a PME ligand-binding cleft structure. PMID: 26183897
    3. Transgenic lines overexpressing the DUF642 proteins encoded by At4g32460 and At5g11420 showed increased pectin methyl esterase 3 activity during seed germination, and improved seed germination performance. PMID: 25442819
    4. role of pectin methyl esterase and preference in wall-associated kinase-activated, EDS1/PAD4-dependent stress response in Arabidopsis PMID: 24855660
    5. Ectopic expression of AtPME3 causes strong Zn(2+) hypersensitivity that is tightly correlated with transcript abundance. Together these observations suggest detrimental effects on Golgi-localized processes. PMID: 23826687
    6. The biochemical characterization of the Arabidopsis thaliana pectin methylesterase 3 gene (AtPME3; At3g14310) and its role in plant development, is reported.[PME3] PMID: 21692803
    7. Upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of AtPME3 that acts as a susceptibility factor and is required for the initial colonization of the host tissue. PMID: 21171891
    8. PME3 directly interacts with the nematode cellulose binding protein which aids cyst nematode parasitism. PMID: 19001564

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  • 亚细胞定位：
    Secreted, extracellular space, apoplast. Secreted, cell wall.
  • 蛋白家族：
    PMEI family; Pectinesterase family
  • 组织特异性：
    Expressed in leaves, stems, flowers and siliques.
  • 数据库链接：

    KEGG: ath:AT3G14310

    STRING: 3702.AT3G14310.1

    UniGene: At.43283

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