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  4. Recombinant Arabidopsis thaliana LRR receptor-like serine/threonine-protein kinase EFR (EFR), partial

Recombinant Arabidopsis thaliana LRR receptor-like serine/threonine-protein kinase EFR (EFR), partial

  • 中文名称：
    拟南芥EFR重组蛋白
  • 货号：
    CSB-YP495669DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥EFR重组蛋白
  • 货号：
    CSB-EP495669DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥EFR重组蛋白
  • 货号：
    CSB-EP495669DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥EFR重组蛋白
  • 货号：
    CSB-BP495669DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥EFR重组蛋白
  • 货号：
    CSB-MP495669DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EFR
  • Uniprot No.：
    C0LGT6
  • 别名：
    EFR; At5g20480; F7C8.70LRR receptor-like serine/threonine-protein kinase EFR; EC 2.7.11.1; Elongation factor Tu receptor; EF-Tu receptor
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Constitutes the pattern-recognition receptor (PPR) that determines the specific perception of elongation factor Tu (EF-Tu), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs); phosphorylates BIK1 upon elicitation to regulate immune responses such as defense hormone expression (e.g. jasmonic acid (JA) and salicylic acid (SA)). Reduces transformation by Rhizobium radiobacter probably by inducing plant defense during the interaction. Binding to the effector AvrPto1 from P.syringae blocks the downstream plant immune response while interaction with hopD2 decreases the phosphorylation level of EFR upon elf18 treatment. Specific endoplasmic reticulum quality control components (ERD2B, CRT3, UGGT and STT3A) are required for the biogenesis of EFR.
  • 基因功能参考文献：
    1. transgenic rice plants (Oryza sativa L. ssp. japonica cv. Zhonghua 17) and cell cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. PMID: 25358295
    2. When challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzae, transgenic EFR wheat lines had reduced lesion size and bacterial multiplication. PMID: 25760815
    3. OST3/6 deficiency affects the biogenesis of EFR, resulting in the impairment of innate immunity. OST3/6 plays an important role for abiotic stress tolerance and for the functionality of the endoglucanase KOR1 essential for cellulose biosynthesis. PMID: 23493405
    4. A knockout mutant showed reduced downy mildew infection, but susceptibility was fully restored through complementation of the mutation, suggesting that the (malectin like)LRR-RLK contributes to disease. PMID: 21711359
    5. The effects of protein domain mutations on the binding properties of EFR and FLS2 are reported. PMID: 21063169
    6. The ligand-induced heteromerization of EFR and FLS2 with BAK1 is independent of kinase activity in planta. PMID: 21593986
    7. Activation of FLS2 and EFR by bacterial peptide elicitors led to calcium-associated plasma membrane anion channel opening. PMID: 20113440
    8. EFR(N143Q) lacking a single conserved N-glycosylation site from the EFR ectodomain accumulated to reduced levels and lost the ability to bind its ligand and to mediate elf18-elicited oxidative burst. PMID: 20007973
    9. These results show that the plasma membrane pattern-recognition receptor EFR requires the endoplasmic reticulum complex SDF2-ERdj3B-BiP for its proper accumulation. PMID: 19763086

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: ath:AT5G20480

    STRING: 3702.AT5G20480.1

    UniGene: At.31176

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