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  4. Recombinant Arabidopsis thaliana Indole-3-acetic acid-amido synthetase GH3.5 (GH3.5), partial

Recombinant Arabidopsis thaliana Indole-3-acetic acid-amido synthetase GH3.5 (GH3.5), partial

  • 中文名称：
    拟南芥GH3.5重组蛋白
  • 货号：
    CSB-YP526844DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥GH3.5重组蛋白
  • 货号：
    CSB-EP526844DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥GH3.5重组蛋白
  • 货号：
    CSB-EP526844DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥GH3.5重组蛋白
  • 货号：
    CSB-BP526844DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥GH3.5重组蛋白
  • 货号：
    CSB-MP526844DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GH3.5
  • Uniprot No.：
    O81829
  • 别名：
    GH3.5; At4g27260; M4I22.70Indole-3-acetic acid-amido synthetase GH3.5; EC 6.3.2.-; Auxin-responsive GH3-like protein 5; AtGH3-5
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin. Strongly reactive with Glu, Gln, Trp, Asp, Ala, Leu, Phe, Gly, Tyr, Met, Ile and Val. Little or no product formation with His, Ser, Thr, Arg, Lys, or Cys. Also active on pyruvic and butyric acid analogs of IAA, PAA and the synthetic auxin naphthaleneacetic acid (NAA). The two chlorinated synthetic auxin herbicides 2,4-D and 3,6-dichloro-o-anisic acid (dicamba) cannot be used as substrates.
  • 基因功能参考文献：
    1. This study suggests that AtGH3.5 conjugates auxins (i.e., IAA and PAA) and benzoates (i.e., SA and BA) to mediate crosstalk between different metabolic pathways, broadening the potential roles for GH3 acyl acid amido synthetases in plants. PMID: 27849615
    2. Arabidopsis acetyl-amido synthetase GH3.5 involvement in camalexin biosynthesis through conjugation of indole-3-carboxylic acid and cysteine and upregulation of camalexin biosynthesis genes. PMID: 22624950
    3. GH3.5 gene is required for fine-tuning adventitious root initiation in the Arabidopsis thaliana hypocotyl, and is acting by modulating jasmonic acid homeostasis PMID: 22730403
    4. Results provide support for WES1 regulating hypocotyl growth by mediating phytochrome B-perceived light signals. PMID: 17602188
    5. This study shows that GH3.5 acts as a bifunctional modulator in both salicylic acid and auxin signaling during pathogen infection. PMID: 17704230

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  • 蛋白家族：
    IAA-amido conjugating enzyme family
  • 数据库链接：

    KEGG: ath:AT4G27260

    STRING: 3702.AT4G27260.1

    UniGene: At.2338

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