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  4. Recombinant Arabidopsis thaliana Histone acetyltransferase GCN5 (HAG1), partial

Recombinant Arabidopsis thaliana Histone acetyltransferase GCN5 (HAG1), partial

  • 中文名称：
    拟南芥HAG1重组蛋白
  • 货号：
    CSB-YP858331DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥HAG1重组蛋白
  • 货号：
    CSB-EP858331DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥HAG1重组蛋白
  • 货号：
    CSB-EP858331DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥HAG1重组蛋白
  • 货号：
    CSB-BP858331DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥HAG1重组蛋白
  • 货号：
    CSB-MP858331DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HAG1
  • Uniprot No.：
    Q9AR19
  • 别名：
    HAG1; BGT; GCN5; HAT1; At3g54610; T14E10.180Histone acetyltransferase GCN5; AtGCN5; EC 2.3.1.48; BIG TOP protein
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acetylates histone H3 and ADA2 proteins in vitro. Acetylates 'Lys-14' of histone H3. Acetylation of histones gives a specific tag for epigenetic transcription activation. Operates in concert with certain DNA-binding transcriptional activators. Acts via the formation of large multiprotein complexes that modify the chromatin.
  • 基因功能参考文献：
    1. required, with ADA2b, in leaves and trichomes to couple nuclear content with cell growth and morphogenesis PMID: 29846775
    2. we find that INO can directly interact with ADA2b/PROPORZ1 (PRZ1), a transcriptional coactivator that is known to interact with the histone acetyltransferase GENERAL CONTROL NONREPRESSIBLE PROTEIN 5 (GCN5, also known as HAG1). PMID: 28971961
    3. signals from the CLV transduction pathway reach the GCN5-containing complexes in the nucleus and alter the histone acetylation status of ethylene-responsive genes. PMID: 26596766
    4. It was concluded that these data indicate that GCN5 plays a key role in the preservation of thermotolerance via versatile regulation in Arabidopsis. PMID: 26576681
    5. Data show that gcn5 mutants display defects in stamen number and arrangement at later stages. PMID: 19771450
    6. The isolation and characterization of EML, a protein that is regulated by GCN5, are reported. PMID: 17151888
    7. the gcn5 mutation seriously reduced acetylation of histone H3K14 and H3K27, whereas the T-DNA insertions of the PP2C6-6 gene enhanced the acetylation of these lysine residues PMID: 18498779
    8. GCN5 associates with a large number of active promoters, independent of the bromodomain that recognizes acetylated histone tails. However, the bromodomain is required for binding subset of promoter regions of some transcription factor genes. PMID: 18644002
    9. GCN5 is essential for root stem cell niche maintenance and acts in the PLETHORA pathway. PMID: 19376933
    10. GCN5-dependent histone acetylation interferes with the miRNA pathways by regulating microRNA production at several levels. PMID: 19436261

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Acetyltransferase family, GCN5 subfamily
  • 组织特异性：
    Expressed in roots and leaves.
  • 数据库链接：

    KEGG: ath:AT3G54610

    STRING: 3702.AT3G54610.1

    UniGene: At.10490

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