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  4. Recombinant Arabidopsis thaliana High affinity nitrate transporter 2.5 (NRT2.5), partial

Recombinant Arabidopsis thaliana High affinity nitrate transporter 2.5 (NRT2.5), partial

  • 中文名称：
    拟南芥NRT2.5重组蛋白
  • 货号：
    CSB-YP864808DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥NRT2.5重组蛋白
  • 货号：
    CSB-EP864808DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥NRT2.5重组蛋白
  • 货号：
    CSB-EP864808DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥NRT2.5重组蛋白
  • 货号：
    CSB-BP864808DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥NRT2.5重组蛋白
  • 货号：
    CSB-MP864808DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NRT2.5
  • Uniprot No.：
    Q9LPV5
  • 别名：
    NRT2.5; At1g12940; F13K23.20; High affinity nitrate transporter 2.5; AtNRT2:5
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nitrate transporter involved in the constitutive high-affinity transport system (cHATS) under long-term N starvation conditions. Predominantly expressed in roots of nitrate-deprived plants as a 150 kDa molecular complex with NRT3.1 representing the major contributor to cHATS influx. The principal role of this cHATS is to enable roots previously deprived of nitrate to absorb this ion and initiate induction of nitrate-inducible genes. Not involved in transfer of nitrate from roots to shoots. Contributes to phloem loading of nitrate in shoots during N starvation, but not required for growth and nitrate uptake in young plants. Required for the nitrate uptake-independent plant growth promotion and lateral root response to the rhizospheric Phyllobacterium. Might be involved in the transfer of nitrate from stored pools to cytoplasm.
  • 基因功能参考文献：
    1. AtNRT2.5 is predominantly expressed in roots of nitrate-deprived WT plants as a 150 kDa molecular complex with AtNAR2.1. Estimates of the kinetic properties of the NRT2.5 transporter reveal that its low Km for nitrate makes this transporter ideally suited to detect and respond to trace quantities of nitrate in the root environment. [AtNRT2.5] PMID: 25474587
    2. Growth analyses of multiple mutants between NRT2.1, NRT2.2, NRT2.4, and NRT2.5 revealed that NRT2.5 is required to support growth of nitrogen-starved adult plants by ensuring the efficient uptake of nitrate collectively with NRT2.1, NRT2.2 and NRT2.4 PMID: 25065551
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Nitrate/nitrite porter (TC 2.A.1.8) family
  • 组织特异性：
    Expressed in roots, shoots and seeds. Expressed in leaves. Expressed in root hair zone of the primary root and the lateral roots, but not in the lateral root tip or in older parts of the roots. Detected mainly in the epidermis and the cortex. Expressed in
  • 数据库链接：

    KEGG: ath:AT1G12940

    STRING: 3702.AT1G12940.1

    UniGene: At.42054

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