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  4. Recombinant Arabidopsis thaliana High-affinity nitrate transporter 2.1 (NRT2.1), partial

Recombinant Arabidopsis thaliana High-affinity nitrate transporter 2.1 (NRT2.1), partial

  • 中文名称：
    拟南芥NRT2.1重组蛋白
  • 货号：
    CSB-YP525775DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥NRT2.1重组蛋白
  • 货号：
    CSB-EP525775DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥NRT2.1重组蛋白
  • 货号：
    CSB-EP525775DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥NRT2.1重组蛋白
  • 货号：
    CSB-BP525775DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥NRT2.1重组蛋白
  • 货号：
    CSB-MP525775DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NRT2.1
  • Uniprot No.：
    O82811
  • 别名：
    NRT2.1; ACH1; LIN1; NRT2;1AT; At1g08090; T6D22.17; High-affinity nitrate transporter 2.1; AtNRT2:1; Protein ACH1; Protein LATERAL ROOT INITIATION 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in nitrate transport, but does not seem to be able to mediate transport by its own. Acts as a dual component transporter with NTR3.1. Acts as a repressor of lateral root initiation under high sucrose/low nitrate conditions.
  • 基因功能参考文献：
    1. A major role for the high-affinity nitrate transporter NRT2.1 in increasing root hydraulic conductivity PMID: 26823528
    2. Glucose elevates NRT2.1 protein levels. PMID: 24272701
    3. Data indicate that leaf nitrogen concentration was lower in mutant nitrate transporter nrt2 than the wild type but was not different for mutant nitrate reductase 2 (nia2) and nitrate reductase 1 (nia1). PMID: 23414092
    4. deletion in the high affinity nitrate trasporter NRT2.1 in Arabidopsis results in a reduced susceptibility to Pseudomonas syringae. PMID: 22580578
    5. Results show that, in the atnrt2 mutant lacking both AtNRT2.1 and AtNRT2.2, gene functions present different kinetic parameters on the NH(4)(+) ion influx mediated by the HATS, according to the source and availability of nitrogen. PMID: 22137605
    6. conclude from this study that posttranscriptional regulation of NRT2.1 and/or NAR2.1 plays a predominant role in the control of the NO(3)(-) HATS in Arabidopsis PMID: 22158677
    7. NRT2.1 has a direct stimulatory role in lateral root development and coordinates root development with external NO(3)(-) availability. PMID: 16415211
    8. Repression of NRT2.1 and NRT3.1 by high nitrates is specifically mediated by the NRT1.1 transporter. PMID: 16998085
    9. The function of NRT2.1 and NAR2.1 as a two-component system transporting nitrates in A. thaliana is reported. [AtNRT2.1] PMID: 17012411
    10. NRT2.1 has a major role in the inducible high-affinity transport system. PMID: 17085507
    11. NRT2.1 has a role in regulating root nitrate uptake in Arabidopsis thaliana PMID: 17573350
    12. the AtNRT2.1 protein is located in the plasma membrane of the root cells PMID: 17583518
    13. The activity of a cis-acting element in the NRT2.1 gene of A. thaliana is described and discussed. PMID: 17897408

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Nitrate/nitrite porter (TC 2.A.1.8) family
  • 组织特异性：
    Root cortical and epidermal cells. Not expressed in new lateral root Primordia. Detected in shoots.
  • 数据库链接：

    KEGG: ath:AT1G08090

    STRING: 3702.AT1G08090.1

    UniGene: At.5407

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