Recombinant Arabidopsis thaliana Heat shock protein 21, chloroplastic (HSP21)

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中文名称：

拟南芥HSP21重组蛋白
货号：

CSB-EP327028DOA
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 90% as determined by SDS-PAGE.
基因名：

HSP21
Uniprot No.：

P31170
别名：

25.3 kDa heat shock protein, chloroplastic
种属：

Arabidopsis thaliana (Mouse-ear cress)
蛋白长度：

Full Length of Mature Protein
来源：

E.coli
分子量：

28.4 kDa
表达区域：

44-227aa
氨基酸序列

AQDQRENSIDVVQQGQQKGNQGSSVEKRPQQRLTMDVSPFGLLDPLSPMRTMRQMLDTMDRMFEDTMPVSGRNRGGSGVSEIRAPWDIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTKVERKVIDVQIQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Chaperone protein required for seedling and chloroplast development under heat stress, probably by maintaining plastid-encoded RNA polymerase (PEP)-dependent transcription.
基因功能参考文献：
1. plastid-localized metalloprotease FtsH6 regulates HSP21 abundance. Lack of a functional FtsH6 protein promotes HSP21 accumulation during the later stages of thermomemory and increases thermomemory capacity. PMID: 27561243
2. Findings reveal an auto-adaptation loop pathway that plant cells optimize particular needs of chloroplasts in stabilizing photosynthetic complexes by relaying the GUN5-dependent plastid signal(s) to activate the heat-responsive expression of HSP21 in the nucleus during adaptation to heat stress in plants. PMID: 27943531
3. Studied Hsp21 association with thylakoid membranes in heat stress resistance of Arabidopsis thaliana. PMID: 28608391
4. analysis of the structure of dodecameric heat-shock protein Hsp21 PMID: 28325834
5. Present a structure model of Arabidopsis thaliana Hsp21, obtained by homology modeling, single-particle electron microscopy, and lysine-specific chemical crosslinking. PMID: 21280121
6. evolution of a pseudogene: diversity and divergence of HSP21 PMID: 18356953
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亚细胞定位：

Plastid, chloroplast stroma, chloroplast nucleoid.
蛋白家族：

Small heat shock protein (HSP20) family
组织特异性：

Confined to pollen grains of budding flowers.
数据库链接：

KEGG: ath:AT4G27670

STRING: 3702.AT4G27670.1

UniGene: At.5368

Recombinant Arabidopsis thaliana Heat shock protein 21, chloroplastic (HSP21)

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