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  4. Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)

Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)

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  • 中文名称：
    拟南芥GAPC1重组蛋白
  • 货号：
    CSB-EP326578DOA
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    GAPC1
  • Uniprot No.：
    P25858
  • 别名：
    NAD-dependent glyceraldehydephosphate dehydrogenase C subunit 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    44.4 kDa
  • 表达区域：
    1-338aa
  • 氨基酸序列
    MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKHNELKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHSITATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYDEIKKAIKEESEGKLKGILGYTEDDVVSTDFVGDNRSSIFDAKAGIALSDKFVKLVSWYDNEWGYSSRVVDLIVHMSKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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GAPC1 Proteins

靶点详情

  • 功能：
    Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism. Required for full fertility. Involved in response to oxidative stress by mediating plant responses to abscisic acid (ABA) and water deficits through the activation of PLDDELTA and production of phosphatidic acid (PA), a multifunctional stress signaling lipid in plants. Associates with FBA6 to the outer mitochondrial membrane, in a redox-dependent manner, leading to binding and bundling of actin. Actin binding and bundling occurs under oxidizing conditions and is reversible under reducing conditions. May be part of a redox-dependent retrograde signal transduction network for adaptation upon oxidative stress. Binds DNA in vitro.
  • 基因功能参考文献：
    1. the redox-dependent localization of GapC1 and GapC2 in the different cellular subcompartments and their interactions with VDAC3 and Trx-h3 PMID: 30189844
    2. The E3 ubiquitin-ligase SEVEN IN ABSENTIA like 7 mono-ubiquitinates GAPC1 in vitro and is required for its nuclear localization. PMID: 26582368
    3. After perception of bacterial flagellin, GAPC1 dynamically responded with a significant increase in size of fluorescent puncta and enhanced nuclear accumulation PMID: 25918875
    4. FERONIA controls leaf starch accumulation by interacting with GAPC1. PMID: 26232644
    5. GAPC levels play important roles in the overall cellular production of reductants, energy, and carbohydrate metabolites and that GAPC levels are directly correlated with seed oil accumulation. PMID: 24989043
    6. Glycolytic GAPC1, through its high sensitivity to the cellular redox state, may play a role in oxidative stress signaling or protection in plants. PMID: 23569110
    7. Results indicate that the H(2)O(2)-promoted interaction of GAPC and PLDdelta may provide a direct connection between membrane lipid-based signaling, energy metabolism and growth control in the plant response to ROS and water stress. PMID: 22589465
    8. The addition of GSSG, and much more efficiently of S-nitrosoglutathione, was shown to inactivate the enzymes GapC1 and GapC2 from Arabidopsis thaliana, spinach, yeast and rabbit muscle. [GapC1] PMID: 18298409

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Glyceraldehyde-3-phosphate dehydrogenase family
  • 组织特异性：
    Expressed in leaves, stems and siliques and at lower levels in roots and flowers.
  • 数据库链接：

    KEGG: ath:AT3G04120

    STRING: 3702.AT3G04120.1

    UniGene: At.22963

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