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  4. Recombinant Arabidopsis thaliana Glutamate--cysteine ligase, chloroplastic (GSH1)

Recombinant Arabidopsis thaliana Glutamate--cysteine ligase, chloroplastic (GSH1)

  • 中文名称：
    拟南芥GSH1重组蛋白
  • 货号：
    CSB-YP343567DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥GSH1重组蛋白
  • 货号：
    CSB-EP343567DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥GSH1重组蛋白
  • 货号：
    CSB-EP343567DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥GSH1重组蛋白
  • 货号：
    CSB-BP343567DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥GSH1重组蛋白
  • 货号：
    CSB-MP343567DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GSH1
  • Uniprot No.：
    P46309
  • 别名：
    GSH1; CAD2; GCL; PAD2; RML1; At4g23100; F7H19.290; Glutamate--cysteine ligase; chloroplastic; EC 6.3.2.2; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Protein ROOT MERISTEMLESS 1; AtGCL; Protein cadmium-sensitive 2; Protein phytoalexin-deficient 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    -
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Seems to play an important role in controlling the expression of resistance responses like the regulation of salicylic acid (SA) and phytoalexin (camalexin) production. Involved in resistance to fungal and bacterial pathogens. Required for the regulation of cell proliferation in root apical meristems through the GSH-dependent developmental pathway. Also participates in the detoxification process, the antioxidant response and is essential for embryo development and proper seed maturation.
  • 基因功能参考文献：
    1. The pad2-1 mutation is related to a decrease in GCL protein and that the resulting glutathione deficiency negatively affects important processes of disease resistance. PMID: 22007023
    2. Glutathione is a key regulator of GSH1 gene expression in response to low temperature. PMID: 22628560
    3. GSH1 is involved in the biosynthesis of glutathione. The zir1 mutant is defective in Fe-mediated Zn tolerance. PMID: 22066515
    4. The thiol-based regulation of GCL provides a posttranslational mechanism for modulating enzyme activity in response to in vivo redox environment and suggests a role for oxidative signaling in the maintenance of glutathione homeostasis in plants. PMID: 17766407
  • 亚细胞定位：
    Plastid, chloroplast.
  • 蛋白家族：
    Carboxylate-amine ligase family, Glutamate--cysteine ligase type 2 subfamily
  • 组织特异性：
    Abundant in leaves and roots. Expressed to a high level in leaf trichomes of mature plant.
  • 数据库链接：

    KEGG: ath:AT4G23100

    STRING: 3702.AT4G23100.1

    UniGene: At.25074

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