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  4. Recombinant Arabidopsis thaliana Ethylene-overproduction protein 1 (ETO1), partial

Recombinant Arabidopsis thaliana Ethylene-overproduction protein 1 (ETO1), partial

  • 中文名称：
    拟南芥ETO1重组蛋白
  • 货号：
    CSB-YP528960DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥ETO1重组蛋白
  • 货号：
    CSB-EP528960DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥ETO1重组蛋白
  • 货号：
    CSB-EP528960DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥ETO1重组蛋白
  • 货号：
    CSB-BP528960DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥ETO1重组蛋白
  • 货号：
    CSB-MP528960DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ETO1
  • Uniprot No.：
    O65020
  • 别名：
    ETO1; At3g51770; ATEM1.2Ethylene-overproduction protein 1; Protein ETHYLENE OVERPRODUCER 1; Protein ETO1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential regulator of the ethylene pathway, which acts by regulating the stability of 1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes. May act as a substrate-specific adapter that connects ACS enzymes, such as ACS5, to ubiquitin ligase complexes, leading to proteasomal degradation of ACS enzymes.
  • 基因功能参考文献：
    1. The mutant exhibits enhanced ethylene production via loss of function of ETHYLENE OVERPRODUCER1 (ETO1; whose normal function is to reduce ethylene production by degrading ethylene biosynthetic enzymes). PMID: 24064767
    2. Lack of ETO1 function confers soil-salinity tolerance through improved shoot Na/K homeostasis, effected via the ethylene resistant1-constitutive triple response1 ethylene signaling pathway. PMID: 24064768
    3. Treatment of etiolated Arabidopsis seedlings with light rapidly increases the stability of ACS5 protein and destabilizes the ETO1/EOLs proteins. PMID: 24065059
    4. The level of ETHYLENE-OVERPRODUCER1 (ETO1)/ETO1-LIKE (EOLs) in vivo plays a role in mediating ACS (1-aminocyclopropane-1-carboxylate synthase) protein turnover, with increased levels leading to a decrease in ACS protein levels. PMID: 23512855
    5. In Arabidopsis, ETO1 encodes a negative regulator of ethylene biosynthesis, and mutation of ETO1 causes Arabidopsis seedlings to overproduce ethylene. PMID: 22623414
    6. Ethylene overproduction partially restores auxin responsiveness to auxin-resistant mutants of ETO1. PMID: 21105933
    7. ETO1 (ETHYLENE-OVERPRODUCER1) is a negative regulator of type 2 1-aminocyclopropane-1-carboxylate synthases(ACC synthase). [ETO1] PMID: 16091151
    8. ETO1, EOL1 and EOL2 work together to negatively regulate ethylene synthesis, by helping degrade type-2 ACC synthases. PMID: 18808454
    9. In contrast to the wild type and ein3-1 mutants, both etr1-1 and eto1-1 mutants showed a sharp (up to 5-fold) increase in alpha-tocopherol levels during leaf aging. PMID: 19258016

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  • 蛋白家族：
    ETO1 family
  • 组织特异性：
    Predominantly expressed in flowers.
  • 数据库链接：

    KEGG: ath:AT3G51770

    STRING: 3702.AT3G51770.2

    UniGene: At.35356

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