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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase KEG (KEG), partial

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase KEG (KEG), partial

  • 中文名称：
    拟南芥KEG重组蛋白
  • 货号：
    CSB-YP887863DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥KEG重组蛋白
  • 货号：
    CSB-EP887863DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥KEG重组蛋白
  • 货号：
    CSB-EP887863DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥KEG重组蛋白
  • 货号：
    CSB-BP887863DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥KEG重组蛋白
  • 货号：
    CSB-MP887863DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KEG
  • Uniprot No.：
    Q9FY48
  • 别名：
    KEG; At5g13530; T6I14.60; T6I14.70E3 ubiquitin-protein ligase KEG; EC 2.3.2.27; EC 2.7.11.1; Protein KEEP ON GOING; RING finger protein KEG; RING-type E3 ubiquitin transferase KEG
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates E2-dependent protein ubiquitination. Acts as a negative regulator of abscisic acid signaling. Required for ABI5 degradation, by mediating its ubiquitination. Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses.
  • 基因功能参考文献：
    1. KEG is capable of attaching ubiquitin to FDH in in vitro assays and the turnover of FDH was increased when co-expressed with a functional KEG in planta, suggesting that KEG contributes to FDH degradation. PMID: 29270890
    2. KEG is an important factor in plant hormone signaling and a positive regulator of JAZ12 stability. PMID: 26320228
    3. CIPK26(AT5G21326) interacts with KEG is degraded by the ubiquitin-proteasome system. PMID: 23658427
    4. KEG is a key component of trans-Golgi network and early endosomes (TGN/EE) that regulates post-Golgi trafficking events, including vacuole biogenesis, targeting of membrane-associated proteins to the vacuole, and secretion of apoplastic proteins. PMID: 23192225
    5. EDR1 and KEG function together to regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network/early endosome vesicles during stress responses. PMID: 21343429
    6. Overexpression of KEG renders plant insensitive to abscisic acid and salt. Abscisic acid promotes KEG degradation via the ubiquitin-dependent 26S proteasome pathway. PMID: 20682837
    7. KEG is central to abscisic acid signaling by maintaining low levels of ABI5 in the absence of stress. PMID: 17194765
    8. Loss-of-function mutations in KEG are seedling lethal and are hypersensitive to glucose and abscisic acid PMID: 18815384

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  • 亚细胞定位：
    Golgi apparatus, trans-Golgi network. Early endosome.
  • 组织特异性：
    Expressed in all tissues of young seedlings. In flowering plants, only detected in the youngest part of the stem, anthers and the receptacle of immature siliques. Not found in mature leave, older parts of the stem, flower parts other than anthers or matur
  • 数据库链接：

    KEGG: ath:AT5G13530

    STRING: 3702.AT5G13530.1

    UniGene: At.32056

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