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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1 (COP1), partial

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1 (COP1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1(COP1) ,partial
  • 货号：
    CSB-YP337358DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1(COP1) ,partial
  • 货号：
    CSB-EP337358DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1(COP1) ,partial
  • 货号：
    CSB-EP337358DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1(COP1) ,partial
  • 货号：
    CSB-BP337358DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1(COP1) ,partial
  • 货号：
    CSB-MP337358DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    COP1
  • Uniprot No.：
    P43254
  • 别名：
    COP1; At2g32950; T21L14.11; E3 ubiquitin-protein ligase COP1; EC 2.3.2.27; Constitutive photomorphogenesis protein 1; RING-type E3 ubiquitin transferase COP1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that acts as a repressor of photomorphogenesis and as an activator of etiolation in darkness. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Represses photomorphogenesis in darkness by mediating ubiquitination and subsequent proteasomal degradation of light-induced transcription factors such as HY5, HYH and LAF1. Down-regulates MYB21, probably via ubiquitination process. Light stimuli abrogate the repression of photomorphogenesis, possibly due to its localization to the cytoplasm. Could play a role in switching between skotomorphogenetic and photomorphogenetic pathways. Mediates the ubiquitination-dependent degradation of HY5 in the darkness during seedling development (e.g. hypocotyl growth). Represses CIP7 in darkness.
  • 基因功能参考文献：
    1. COL12 is a substrate of the COP1. PMID: 29187570
    2. plays a prominent role in drought stress tolerance PMID: 30139551
    3. Mutation of the coiled-coil domain in COP1, which prevents dimer formation, impairs COP1 function in coordinating flowering time. PMID: 29273730
    4. Nitrate Reductases levels are negatively regulated by COP1 and ammonium. PMID: 29662028
    5. Data indicate that light is directly linked with the inducer of CBF Expression (ICE) transcription factors (ICE)-directed signaling module, via the CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-mediated protein surveillance system, in the modulation of stomatal development. PMID: 29070509
    6. COP1/SPA complex associates with and stabilizes PHYTOCHROME INTERACTING FACTOR 3 (PIF3) to repress photomorphogenesis in the dark. PMID: 28292892
    7. High COP1 expression is associated with defense against turnip crinkle virus. PMID: 29513740
    8. COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation. PMID: 29087315
    9. Thermal activation of COP1 enables coincidence between warm temperature signaling and circadian rhythms, which allows plants to gate hypocotyl thermomorphogenesis at the most profitable time at warm temperatures. PMID: 28418582
    10. ABI4 and HY5 antagonistically regulate the expression of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) and the subsequent greening process. In turn, ABI4 and HY5 are targeted for degradation by COP1 in the light and dark, respectively, to ensure a proper interplay of ABI4 and HY5 actions during seedling de-etiolation. PMID: 27255835
    11. Data indicate localization of UVR8 signaling in the nucleus and a dual role for COP1 in the regulation of UV-B-induced UVR8 nuclear accumulation and in UVR8-mediated UV-B signaling. PMID: 27407149
    12. SPA proteins have a dual role: (1) they are required for light-responsiveness of COP1 subcellular localization, and (2) they promote COP1 activity in darkness in a fashion that is independent of the nuclear import/nuclear retention of COP1. PMID: 28536102
    13. The alpha2-cop mutant displayed defects in plant growth, including small rosettes, stems and roots and mislocalization of p24delta5, a protein of the p24 family containing a C-terminal dilysine motif involved in COPI binding. The alpha2-cop mutant also exhibited abnormal morphology of the Golgi apparatus. PMID: 28025315
    14. Exposure to blue light is required for an in vivo-association of CRY1 and CRY2 with COP1. PMID: 28991901
    15. DHU1 negatively regulates UV-B signaling via its direct interaction with COP1 and RUP1 (At5g52250). PMID: 28735869
    16. BBX21 is a pivotal component involved in the COP1-HY5 regulatory hub. PMID: 27325768
    17. Meanwhile, transcript levels of ABA biosynthesis genes are higher in cip1-1 than in the wild-type. These results suggested that CIP1 is positively involved in ABA response. PMID: 27372427
    18. salt stress and ethylene antagonistically regulate nucleocytoplasmic partitioning of COP1. PMID: 26850275
    19. Our genetic and biochemical studies identify a function for SIZ1 in photomorphogenesis and reveal a novel SUMO-regulated ubiquitin ligase, COP1, in plants. PMID: 27128446
    20. Data indicate a coordinated regulation of Arabidopsis proteins SHW1, COP1, and HY5 in seedling development. PMID: 26474641
    21. The COP1 role in CONSTANS protein degradation during photoperiodic flowering PMID: 26358558
    22. CSU2 interacts with COP1 via the coiled-coil domain association. CSU2 negatively regulates COP1 E3 ubiquitin ligase activity. [COP1] PMID: 26714275
    23. propose that light perceived by phytochromes causes a switch in the ubiquitination activity of COP1/SPA2 from ubiquitinating downstream substrates to ubiquitinating SPA2, which subsequently causes a repression of COP1/SPA2 function PMID: 26368289
    24. CUL4(COP1-SPA) E3 ubiquitin ligase is necessary for the light-induced degradation of PIF1 in Arabidopsis. PMID: 26037329
    25. genetic analyses with transgenes expressing a genomic pmARI12:ARI12-GFP construct confirm the epistatic interaction between COP1 and ARI12 in growth responses to high fluence rate UV-B. PMID: 25817546
    26. AN3 may act with other proteins that bind to COP1 promoter to promote anthocyanin accumulation and inhibit light-induced root elongation. PMID: 25256341
    27. Two distinct domains of the UVR8 photoreceptor interact with COP1 to initiate UV-B signaling in Arabidopsis PMID: 25627067
    28. cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. PMID: 25532508
    29. It was shown show that mutations in the key repressor of light signaling, the COP1/SPA complex, cause a strong hyperaccumulation of anthocyanins not only under normal light but also under high light conditions. PMID: 25482806
    30. Light and COP1 regulate level of overexpressed DET1 protein PMID: 25575996
    31. Analysis of BBX22 degradation kinetics shows that it has a short half-life under both dark and light conditions. COP1 mediates the BBX22 degradation in the dark. Although dispensable in the dark, HY5 contributes to the BBX22 degradation in the light. PMID: 21427283
    32. COP1 is revealed as a potential coordinator of cytoskeletal and electrophysiological activities required for guard cell function PMID: 25151660
    33. Based on our molecular and biochemical evidence, we propose that the UVR8-COP1 affinity in plants critically determines the photomorphogenic UV-B signal transduction coupling with UVR8-mediated UV-B light perception. PMID: 24651064
    34. COP1 inactivation involved in rapid light-induced responses were compared to that of nuclear HY5. PMID: 24434030
    35. our investigation demonstrates that the COP1-HY5 complex is a novel integrator that plays an essential role in ethylene-promoted hypocotyl growth in the light. PMID: 24348273
    36. The MID protein plays a role in COP1/SPA1-controlled repression of flowering under short-day conditions. PMID: 23857347
    37. COP1 plays a role in setting the daily patterns of sensitivity to shade in the fluctuating light environments of plant canopies. PMID: 23647163
    38. A functional connection between COP1 and the TOPOVI in plants links COP1-dependent development with the regulation of endoreduplication. PMID: 23573936
    39. The COP1/SPA complex affects PAP1 and PAP2 both transcriptionally and post-translationally. The COP1/SPA complex controls anthocyanin levels in Arabidopsis. PMID: 23425305
    40. UV-B-induced reorganization of COP1 complexes achieves a functional switch of COP1 from repressing to promoting photomorphogenesis. PMID: 24067658
    41. the light-induced decline of phyA levels is reduced in spa mutants irrespective of the growth medium, suggesting a COP1-independent role for SUppressor of phytochrome A proteins. PMID: 23391578
    42. In cop1 mutants, ddb1b-2 enhanced the cop1-4 short hypocotyl phenotype in dark and light, enhanced anthocyanin levels in cop1-1 in the light, but had no effect in adult plants. PMID: 23450167
    43. Ala mutants of these tryptophans appear monomeric and constitutively bind COP1 in plants, but their responses indicate that monomer formation and COP1 binding are not sufficient for UVR8 function. PMID: 23012433
    44. COP1 gene expression in response to photomorphogenic UV-B is controlled by a combinatorial regulation of FHY3 and HY5, and this UV-B-specific working mode of FHY3 and HY5 is distinct from that in far-red light and circadian conditions. PMID: 23150635
    45. COP1/SPA activity, via LONG HYPOCOTYL IN FR LIGHT1, is required for shade-induced modulation of the auxin biosynthesis pathway and thereby enhances cell elongation in low red:far red. PMID: 23093358
    46. Ethylene-promoting hypocotyl via IAA is mediated by light, and COP1 has a significant impact on the transcription of some genes downstream of EIN3. Thus, COP1 plays a crucial role in the opposite effects of ethylene on hypocotyl elongation. PMID: 22890836
    47. Mediator component cooperates with COP1 in the regulation of light responses and that the hypersensitive seedling phenotype strictly depends on the presence of HY5, an important positive regulator of light-dependent gene expression. PMID: 22760208
    48. COP1 mediates the coordination of root and shoot growth by light through modulation of PIN1- and PIN2-dependent auxin transport in Arabidopsis PMID: 22912415
    49. COP1 interacts with BBX24 in vivo in a UV-B-dependent manner, and this requires UV-B-induced COP1 accumulation. PMID: 22410790
    50. Mutations in the region responsible for the interaction with COP1 revealed that a physical interaction of the proteins is also required for degradation of BBX24 in the light and for normal photomorphogenesis. PMID: 21685177

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 数据库链接：

    KEGG: ath:AT2G32950

    STRING: 3702.AT2G32950.1

    UniGene: At.298

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