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  4. Recombinant Arabidopsis thaliana E3 SUMO-protein ligase SIZ1 (SIZ1), partial

Recombinant Arabidopsis thaliana E3 SUMO-protein ligase SIZ1 (SIZ1), partial

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  • 中文名称：
    拟南芥SIZ1重组蛋白
  • 货号：
    CSB-EP721197DOA
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    SIZ1
  • Uniprot No.：
    Q680Q4
  • 别名：
    E3 SUMO-protein transferase SIZ1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    23.2 kDa
  • 表达区域：
    1-171aa
  • 氨基酸序列
    MDLEANCKEKLSYFRIKELKDVLTQLGLSKQGKKQELVDRILTLLSDEQAARLLSKKNTVAKEAVAKLVDDTYRKMQVSGASDLASKGQVSSDTSNLKVKGEPEDPFQPEIKVRCVCGNSLETDSMIQCEDPRCHVWQHVGCVILPDKPMDGNPPLPESFYCEICRLTRAD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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SIZ1 Proteins

靶点详情

  • 功能：
    E3 SUMO protein ligase involved in regulation processes. Mediates SUMO/ attachment to PHR1, a MYB transcriptional activator controlling the phosphate deficiency responses. Functions as an upstream negative regulator of salicylic acid (SA) accumulation and subsequent SA-mediated systemic acquired resistance (SAR) signaling. Probably not involved in jasmonic acid (JA)-mediated defense response. Participates in abiotic stress-induced sumoylation. Controls heat shock-induced SUMO1 and SUMO2 conjugation and facilitates basal thermotolerance. Involved in freezing tolerance by mediating sumoylation of ICE1, a transcription activator of the cold signaling regulator CBF3/DREB1A. Acts as positive regulator of drought stress tolerance. Acts as floral repressor that promotes FLC expression by repressing FLD activity through sumoylation. Acts as negative regulator of abscisic acid (ABA) signaling through ABI5 sumoylation. Mediates sumoylation of SCE1, GTE3 and GTE5. Functions as negative regulator of SnRK1 signaling through sumoylation of several components of the SnRK1 complex.
  • 基因功能参考文献：
    1. Arabidopsis thaliana SIZ1 controls nuclear localization of Nitrate Reductases proteins, and ammonium negatively regulates their levels. PMID: 29662028
    2. SIZ1 suppresses an SNC1-dependent resistance response at both normal and high temperatures. At the same time, SIZ1 amplifies the dark and high temperature growth response, likely via COP1 and upstream of gene regulation by PIF4 and BRZ1. PMID: 29357355
    3. The SnRK1 complex is SUMOylated on multiple subunits and identify SIZ1 as the E3 Small Ubiquitin-like Modifier (SUMO) ligase responsible for this modification. PMID: 26662259
    4. regulates seed dormancy and germination PMID: 27130140
    5. SUMO-conjugated proteins accumulate in response to high doses of sugar in a SIZ1-dependent manner. PMID: 26468507
    6. The methylation level of the Arabidopsis genome, including transposons, was significantly lower in the siz1-2 mutant than in wild-type plants. CMT3 was sumoylated by the E3 ligase activity of AtSIZ1 through a direct interaction. PMID: 26398805
    7. negatively affects stomatal closure and drought tolerance through accumulation of salicylic acid PMID: 22963672
    8. This study establishes that the diverse properties characteristic of SIZ1 are associated with specific domains and that they can be separated. PMID: 20404572
    9. The AtSIZ1 mutation is correlated with an overall decrease in alternative respiration and with a low carbohydrate content to maintain the carbon to nitrogen ratio in siz1-2 mutants. PMID: 22732219
    10. SIZ1 sustains the stability and normal function of the mature female gametophyte which is necessary for pollen tube guidance. PMID: 22253727
    11. The expression and enzymatic activity of Cu/ZnSOD1 (CSD1) in shoots of the siz1 mutant under excess Cu, was examined. PMID: 21897129
    12. These data suggest that SIZ1-mediated sumoylation is involved specifically in copper homeostasis and tolerance in planta. PMID: 21632972
    13. AtSIZ1 positively controls nitrogen assimilation by promoting sumoylation of nitrate reductases in Arabidopsis. PMID: 21772271
    14. SIZ1 facilitates the auxin-regulated root architecture remodeling that occurs in response to Pi deficiency. PMID: 21156857
    15. The salicylic acid-accumulating mutant siz1 exhibits sensitivity to chilling and freezing conditions. PMID: 19959255
    16. These results indicate that SIZ1 regulates cell growth and plant development with regulation of salicylic acid accumulation. PMID: 20007967
    17. The various domains of SIZ1 make unique contributions to the plant's ability to cope with its environment. PMID: 19837819
    18. SIZ1 and sumoylation facilitate basal thermotolerance through processes that are salicylic acid independent. PMID: 17041025
    19. SIZ1 is required for salicylic acid and PAD4-mediated R gene signalling, which in turn confers innate immunity in Arabidopsis.[SIZ1] PMID: 17163880
    20. SIZ1 is a SUMO E3 ligase that facilitates conjugation of SUMO to protein substrates. PMID: 17416732
    21. SIZ1 regulates Arabidopsis growth and that this SUMO E3 ligase plays a role in drought stress response likely through the regulation of gene expression. PMID: 17905899
    22. Results suggest that SIZ1 is a floral repressor that not only represses the SA-dependent pathway, but also promotes FLC expression by repressing FLD activity through sumoylation, which is required for full FLC expression in a FRIGIDA background. PMID: 18069938

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  • 亚细胞定位：
    Nucleus speckle.
  • 蛋白家族：
    PIAS family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: ath:AT5G60410

    STRING: 3702.AT5G60410.2

    UniGene: At.43746

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