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  4. Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 2 (DRB2)

Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 2 (DRB2)

  • 中文名称：
    拟南芥DRB2重组蛋白
  • 货号：
    CSB-YP871101DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥DRB2重组蛋白
  • 货号：
    CSB-EP871101DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥DRB2重组蛋白
  • 货号：
    CSB-EP871101DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥DRB2重组蛋白
  • 货号：
    CSB-BP871101DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥DRB2重组蛋白
  • 货号：
    CSB-MP871101DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DRB2
  • Uniprot No.：
    Q9SKN2
  • 别名：
    DRB2; At2g28380; T1B3.10Double-stranded RNA-binding protein 2; dsRNA-binding protein 2; AtDRB2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-434
  • 氨基酸序列
    MYKNQLQELA QRSCFNLPSY TCIREGPDHA PRFKATVNFN GEIFESPQYC STLRQAEHSA AEVALNALSN RGPSHSLAAR ILDETGVYKN LLQEIAQRVG APLPRYTTFR SGLGHQPVFT GTVELAGITF TGDPAKNKKQ AEKNAAMAAW SSLKQLAKET SSSMPEPENI DELEQVIIAR ALINYRIKEN IGTGSSSSAP VPFAKKFFMQ NLRPTSPQPS PATTSRILPF ICPKQPSRSS RSSLAATSGI DRIMAAALES RSYQRPQQRF ANPPYVPMRQ FRSQCHGMAP PVTIRTAVPV FSAPPMPPPP CTNNTQLPSS VYVPSLMRTA PPVRIAPPVT IRTAVPVFAS APPVRIRTAV KPTVEAGETR ISSVQEKESI PVLPDSLEIG VEGSTITITD CEKTASKETE RAEFKDSSKG EPETARERLE NLKI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds double-stranded RNA. May be involved in RNA-mediated silencing.
  • 基因功能参考文献：
    1. DRB2-associated translational inhibition appears to be less ubiquitous and specifically aimed toward responses against abiotic or biotic stimuli. PMID: 26387911
    2. DRB2 is part of a repressive epigenetic regulator complex involved in a negative feedback loop, adjusting epigenetic state to transcription level at transposable element loci. PMID: 25849103
    3. Unlike DRB2, DRB3 and DRB5 are not involved in the double stranded RNA processing stages of the microRNA (miRNA) biogenesis pathway, but are required to mediate RNA silencing of target genes of DRB2-associated miRNAs. PMID: 22902697
    4. Examination of different single and multiple drb mutants revealed a clear association between the loss of DRB2 activity and altered accumulation for both the elevated and reduced miRNA classes. PMID: 22545148
    5. show that the double-stranded RNA binding proteins DRB2 and DRB4 are required for proper accumulation of p4-siRNAs. PMID: 21700726

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: ath:AT2G28380

    STRING: 3702.AT2G28380.1

    UniGene: At.13732

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