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  4. Recombinant Arabidopsis thaliana Diacylglycerol O-acyltransferase 1 (DGAT1), partial

Recombinant Arabidopsis thaliana Diacylglycerol O-acyltransferase 1 (DGAT1), partial

  • 中文名称：
    拟南芥DGAT1重组蛋白
  • 货号：
    CSB-YP871108DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥DGAT1重组蛋白
  • 货号：
    CSB-EP871108DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥DGAT1重组蛋白
  • 货号：
    CSB-EP871108DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥DGAT1重组蛋白
  • 货号：
    CSB-BP871108DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥DGAT1重组蛋白
  • 货号：
    CSB-MP871108DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DGAT1
  • Uniprot No.：
    Q9SLD2
  • 别名：
    DGAT1; ABX45; DAGAT; TAG1; At2g19450; F3P11.5Diacylglycerol O-acyltransferase 1; AtDGAT1; EC 2.3.1.20; Protein TRIACYLGLYCEROL 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.
  • 基因功能参考文献：
    1. DGAT1 expression is regulated by MYB96 in the Arabidopsis seeds. PMID: 29660088
    2. The constructed promoter was ligated upstream of the TAG1 gene encoding diacylglycerol acyltransferase 1 and introduced into Arabidopsis. Seeds from transgenic plants carrying AtTAG1 under the control of the chimeric promoter showed increased oil content (up by 1873%) compared with wild-type seeds. PMID: 29874815
    3. This study identifies the first two regulatory genes, WRI1 and DGAT1, that control the synthesis of all tocochromanol forms in seeds, and shows the existence of a metabolic trade-off between lipid and tocochromanol metabolisms. PMID: 29105089
    4. The results suggest that dedifferentiation is associated with Tag1 activation and that CMT3 rather than DDM1 plays a central role in restraining Tag1 activation via inducing gene body DNA methylation. PMID: 27475038
    5. ABI4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress. PMID: 23942253
    6. Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2. PMID: 22233193
    7. ABI4 is essential for the activation of DGAT1 in Arabidopsis seedlings during nitrogen deficiency. PMID: 21515696
    8. PDAT1 and DGAT1 have overlapping functions for triacylglycerol synthesis in both seed and pollen, and that the absence of their function leads not only to a reduction in TAG, but also to critical defects in normal pollen and embryo development. PMID: 20040537

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  • 亚细胞定位：
    Plastid, chloroplast membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily
  • 组织特异性：
    Ubiquitous. Highest expression in young developing seeds.
  • 数据库链接：

    KEGG: ath:AT2G19450

    STRING: 3702.AT2G19450.1

    UniGene: At.20259

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