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  4. Recombinant Arabidopsis thaliana DNA topoisomerase 1 (TOP1), partial

Recombinant Arabidopsis thaliana DNA topoisomerase 1 (TOP1), partial

  • 中文名称：
    拟南芥TOP1A重组蛋白
  • 货号：
    CSB-YP024056DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥TOP1A重组蛋白
  • 货号：
    CSB-EP024056DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥TOP1A重组蛋白
  • 货号：
    CSB-EP024056DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥TOP1A重组蛋白
  • 货号：
    CSB-BP024056DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥TOP1A重组蛋白
  • 货号：
    CSB-MP024056DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TOP1A
  • Uniprot No.：
    P30181
  • 别名：
    TOP1A; FAS5; MGO1; TOP1; TOP1ALPHA; At5g55300; MTE17.1; DNA topoisomerase 1 alpha; EC 5.6.2.1; DNA topoisomerase 1; DNA topoisomerase I; Protein FASCIATA 5; Protein MGOUN1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Can complement a TOP1-deficient yeast mutant. Plays a critical role in the maintenance of a regular pattern of organ initiation. Topoisomerases I enzymes (TOP1A and TOP1B) are essential for plant survival. Functions together with the stem cell maintenance gene WUSCHEL (WUS) in stem cell regulation. Required to maintain developmentally regulated gene repression. Functions synergistically with chromatin remodeling factors. Is required for the repression of WUS expression in flower development. Plays a role in polycomb group (PcG) protein-mediated histone H3 trimethylation on 'Lys-27' (H3K27me3) at the WUS gene locus. H3K27me3 induces transcriptional repression of WUS. May assist AGAMOUS (AG) in recruiting PcG proteins to WUS locus. Reduces nucleosome density, especially at genes that are targets of PcG proteins. Plays a role in epigenetic silencing. Involved in RNA-directed DNA methylation (RdDM) by promoting Pol V transcription to generate long non-coding RNA transcripts. Is dispensable for Pol IV-mediated small interfering RNA (siRNA) biogenesis. Promotes transposable element (TE) silencing at endogenous RdDM target loci through histone H3 dimethylation of 'Lys-9' (H3K9me2). Promotes the production of Pol V-dependent long non-coding transcripts that facilitate the recruitment of siRNA-AGO4 and AGO4 occupancy at TEs.
  • 基因功能参考文献：
    1. TOP1alpha is required for the survival of stele stem cells and regulates the differentiation state and number of columella stem cells. PMID: 26969721
    2. TOP1ALPHA role in flowering PMID: 27837087
    3. Pleiotropic effect of the fas5 mutation on different stages of ontogeny and different organs suggests that the FAS5 gene plays a complex regulatory role at all stages of the A. thaliana shoot development. [Fas5] PMID: 25898530
    4. Further studies with Arabidopsis top1alpha mutants showed that TOP1alpha silences endogenous RdDM loci by facilitating the production of Pol V-dependent long non-coding RNAs, AGONAUTE4 recruitment and H3K9me2 deposition at TEs and repeats PMID: 24992598
    5. MGO1 encodes a putative Arabidopsis type IB topoisomerase that functions together with WUS in stem cell regulation. [MGO1] PMID: 20228247

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Type IB topoisomerase family
  • 组织特异性：
    Expressed in inflorescence meristems. Expressed in primordia of sepals, petals, stamens, carpels and ovules. Expressed in midstage embryos.
  • 数据库链接：

    KEGG: ath:AT5G55300

    STRING: 3702.AT5G55300.2

    UniGene: At.197

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