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  4. Recombinant Arabidopsis thaliana DNA ligase 1 (LIG1), partial

Recombinant Arabidopsis thaliana DNA ligase 1 (LIG1), partial

  • 中文名称：
    拟南芥LIG1重组蛋白
  • 货号：
    CSB-YP671465DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥LIG1重组蛋白
  • 货号：
    CSB-EP671465DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥LIG1重组蛋白
  • 货号：
    CSB-EP671465DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥LIG1重组蛋白
  • 货号：
    CSB-BP671465DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥LIG1重组蛋白
  • 货号：
    CSB-MP671465DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LIG1
  • Uniprot No.：
    Q42572
  • 别名：
    LIG1; At1g08130; T23G18.1; T6D22.23DNA ligase 1; AtLIG1; EC 6.5.1.1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential protein. DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Involved in repair of both single strand breaks (SSBs) and double strand breaks (DSBs). Required in the endosperm for embryogenesis, probably to repair DNA-breaks generated by DME.
  • 基因功能参考文献：
    1. The AtLIG1 is an important component of the active DNA demethylation machinery. PMID: 25906993
    2. LIG1 is required for both single-nucleotide or long-patch base excision repair. PMID: 21781197
    3. In the study, we show that loss-of-function of the major DNA LIGASE I (AtLIG1) in Arabidopsis thaliana causes maternal effects in the endosperm, which is the seed tissue that nurtures embryo development. PMID: 20023162
    4. Nuclear or mitochondrial targeting of LIG1 is accomplished through an evolutionarily conserved translation initiation mechanism. [AtLIG1] PMID: 16790030
    5. DNA ligase 1 functions in both DNA replication and in repair of both ss and dsDNA strand breaks. PMID: 19558640

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  • 亚细胞定位：
    [Isoform 1]: Mitochondrion.; [Isoform 2]: Nucleus.; [Isoform 3]: Nucleus.
  • 蛋白家族：
    ATP-dependent DNA ligase family
  • 组织特异性：
    Expressed in all vegetative and reproductive tissues.
  • 数据库链接：

    KEGG: ath:AT1G08130

    STRING: 3702.AT1G08130.1

    UniGene: At.36

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