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  4. Recombinant Arabidopsis thaliana DNA (cytosine-5)-methyltransferase DRM2 (DRM2), partial

Recombinant Arabidopsis thaliana DNA (cytosine-5)-methyltransferase DRM2 (DRM2), partial

  • 中文名称：
    拟南芥DRM2重组蛋白
  • 货号：
    CSB-YP873462DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥DRM2重组蛋白
  • 货号：
    CSB-EP873462DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥DRM2重组蛋白
  • 货号：
    CSB-EP873462DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥DRM2重组蛋白
  • 货号：
    CSB-BP873462DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥DRM2重组蛋白
  • 货号：
    CSB-MP873462DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DRM2
  • Uniprot No.：
    Q9M548
  • 别名：
    DRM2; At5g14620/At5g14630; T15N1.110/T15N1.120DNA; cytosine-5)-methyltransferase DRM2; EC 2.1.1.37; Protein DOMAINS REARRANGED METHYLASE 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation (RdDM). Acts as major DNA methyltransferase in the RdDM pathway, and is essential for RNA-directed de novo DNA methylation of cytosines in all sequence contexts. Associates with long non-coding RNA (lncRNA) produced by RNA polymerase V (Pol V). This association is dependent on AGO4 and IDN2, and results in DNA methylation of RdDM target loci.
  • 基因功能参考文献：
    1. CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV PMID: 28115468
    2. DRM2 gene expression throughout normal development in Arabidopsis PMID: 25842863
    3. Study shows that Arabidopsis DRM2 exists in complex with the small interfering RNA (siRNA) effector ARGONAUTE4 (AGO4) and preferentially methylates one DNA strand, likely the strand acting as the template for RNA polymerase V-mediated noncoding RNA transcripts. PMID: 24855943
    4. These findings substantiate the view that DRM2 is the major de novo DNA methyltransferase in the RdDM pathway and support a direct catalytic role for this enzyme in RdDM. PMID: 21212233
    5. Data show that by site-directed mutagenesis that both the DRM2 N-terminal UBA domains and C-terminal methyltransferase domain are required for normal RNA-directed DNA methylation, supporting an essential targeting function for the UBA domains. PMID: 21060858
    6. JMJ14 is specifically involved in the maintenance phase of DRM2-mediated RNA-directed DNA methylation. PMID: 21052090
    7. SRA-domain proteins ( suvh9 and suvh2 ) required for DRM2-mediated de novo DNA methylation PMID: 19043555

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Note=Peri-nucleolar.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, DRM-methyltransferase family
  • 组织特异性：
    Expressed in roots, inflorescences and at lower levels in leaves.
  • 数据库链接：

    KEGG: ath:AT5G14620

    STRING: 3702.AT5G14620.1

    UniGene: At.5084

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