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  4. Recombinant Arabidopsis thaliana DELLA protein RGL2 (RGL2), partial

Recombinant Arabidopsis thaliana DELLA protein RGL2 (RGL2), partial

  • 中文名称：
    拟南芥RGL2重组蛋白
  • 货号：
    CSB-YP812754DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RGL2重组蛋白
  • 货号：
    CSB-EP812754DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RGL2重组蛋白
  • 货号：
    CSB-EP812754DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RGL2重组蛋白
  • 货号：
    CSB-BP812754DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RGL2重组蛋白
  • 货号：
    CSB-MP812754DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RGL2
  • Uniprot No.：
    Q8GXW1
  • 别名：
    RGL2; SCL19; At3g03450; T21P5.13; DELLA protein RGL2; GRAS family protein 15; AtGRAS-15; RGA-like protein 2; Scarecrow-like protein 19; AtSCL19
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Acts as a major GA-response repressor of seed germination, including seed thermoinhibition. Promotes the biosynthesis of abscisic acid (ABA), especially in seed coats to maintain seed dormancy. Delays flowering and adult leaf production. Also regulates the floral development, petal, stamen and anther development, by repressing the continued growth of floral organs. Its activity is probably regulated by other phytohormones such as auxin and ethylene. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination. Promotes salt tolerance. Acts as a repressor of positive regulators of trichome initiation. Required during the flagellin-derived peptide flg22-mediated growth inhibition. Contributes to the susceptibility to the biotrophic pathogen P.syringae pv. tomato and to the resistance to the necrotrophic pathogens B.cinerea A.brassicicola, probably by repressing the SA-defense pathway and preventing cell death. Prevents stress-induced reactive oxygen species (ROS) accumulation (e.g. salt stress) by acting on the ROS scavenging system, and delays ROS-induced cell death, thus promoting stress tolerance.
  • 基因功能参考文献：
    1. NF-YC-RGL2-ABI5 module integrates gibberellic acid and abscisic acid signalling pathways during seed germination PMID: 27624486
    2. RGL2-containing protein complex can bind the promoter of GATA12 and thereby regulate its expression PMID: 28917589
    3. The data indicate that AtGASA6 links RGL2 and AtEXPA1 functions and plays a role as an integrator of gibberellin, abscisic acid, and glucose signaling, resulting in the regulation of seed germination through a promotion of cell elongation. PMID: 26400990
    4. RGL2 directly regulates several genes affecting various physiological aspects to inhibit germination. PMID: 23035751
    5. The persistent high ABA levels in imbibed dormant seeds requires the permanent expression of the DELLA gene RGL2, where it remains insensitive to gibberellins (GA) unlike in nondormant seeds. PMID: 20956298
    6. We confirm that RGL2, one member of the DELLA family, encodes the predominant repressor of seed germination in Arabidopsis and show that the other DELLA genes GAI,RGA and RGL1 enhance the function of RGL2. PMID: 16034591
    7. role of conserved serine and threonine in GA signaling PMID: 16167898
    8. Four conserved tyrosine residues within the RGL2 protein are critical for gibberellin sensitivity in Arabidopsis. PMID: 17333251
    9. RGL2 disappearance is not a prerequisite for seed germination in the sly1 background. PMID: 17384169
    10. RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis PMID: 18941053

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    GRAS family, DELLA subfamily
  • 组织特异性：
    Predominantly expressed in germinating seeds, flowers and siliques. Only detectable in the inflorescence, with high levels in young flower buds and significant levels in siliques. In imbibed seeds, it is restricted to seed coats, elongating regions of pre
  • 数据库链接：

    KEGG: ath:AT3G03450

    STRING: 3702.AT3G03450.1

    UniGene: At.18489

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