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  4. Recombinant Arabidopsis thaliana DELLA protein RGA (RGA), partial

Recombinant Arabidopsis thaliana DELLA protein RGA (RGA), partial

  • 中文名称：
    拟南芥RGA重组蛋白
  • 货号：
    CSB-YP874462DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RGA重组蛋白
  • 货号：
    CSB-EP874462DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RGA重组蛋白
  • 货号：
    CSB-EP874462DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RGA重组蛋白
  • 货号：
    CSB-BP874462DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RGA重组蛋白
  • 货号：
    CSB-MP874462DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RGA
  • Uniprot No.：
    Q9SLH3
  • 别名：
    RGA; GRS; RGA1; At2g01570; F2I9.19; DELLA protein RGA; GAI-related sequence; GRAS family protein 10; AtGRAS-10; Repressor on the ga1-3 mutant; Restoration of growth on ammonia protein 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression in seeds. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Compared to other DELLA proteins, it is the most sensitive to GA application. No effect of the BOI proteins on its stability. Its activity is probably regulated by other phytohormones such as auxin and ethylene, attenuation of auxin transport delaying its GA-induced degradation. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination.
  • 基因功能参考文献：
    1. RGA negatively regulate dark-induced senescence and chlorophyll degradation.RGA physically interacts with WRKY6. PMID: 29574486
    2. The six conserved Ser/Thr sites are important for the different bioactivities of the RGA protein that regulate the gibberellin (GA) response, and also for RGA stability via the mimicking of phosphorylation/de-phosphorylation. PMID: 25056926
    3. RGA and GAI play distinct roles in seed germination. PMID: 25064446
    4. DELLA activity is essential for Arabidopsis pollen development. PMID: 24400898
    5. Data indicate that BZR1 interacts in vitro and in vivo with REPRESSOR OF ga1-3 (RGA), a member of the DELLA family of transcriptional regulators that inhibits the gibberellins (GAs) signaling pathway. PMID: 23033541
    6. RGA represses flowering in leaves through FT and miR172 under long-day conditions, and represses flowering at the shoot apex through MADS box genes. PMID: 22942378
    7. Studies indicate that GRAS proteins are named after the first three members: GIBBERELLIC ACID INSENSITIVE (GAI), REPRESSOR of GAI (RGA) and SCARECROW (SCR), and they have diverse roles in plant development and signal transduction. PMID: 21732203
    8. SPT acts in an analogous manner to the gibberellin-dependent DELLAs, REPRESSOR OF GA1-3 and GIBBERELLIC ACID INSENSITIVE, which restrain cotyledon expansion alongside SPT. PMID: 21478445
    9. We show that repression of germination by far-red light involves stabilized DELLA factors GAI, RGA and RGL2 that stimulate endogenous abscisic acid synthesis which, in turn, blocks germination through the transcription factor ABI3. PMID: 19556968
    10. SLEEPY1 interacts directly with RGA and GA INSENSITIVE (GAI, a closely related DELLA protein) via their C-terminal GRAS domain PMID: 15155881
    11. SLY1 (the wild-type form) and sly1gar2-1 both confer substrate specificity on this complex via specific binding to the DELLA proteins, GAI and RGA PMID: 15161962
    12. We show that ga1-3 seeds lacking RGA, RGL1 and RGL2 or GAI, RGL1 and RGL2, confer GA-independent germination in the light but not in the darkness whilst ga1-3 seeds lacking GAI, RGA and RGL2 germinate both in the light and darkness. PMID: 16034591
    13. DELLA abundance is regulated during growth responses to neighbours in dense Arabidopsis stands. This occurs in a red:far red ratio-dependent manner in petioles, depends on gibberellin, and matches the induction kinetics of petiole elongation. PMID: 17488236
    14. Data show that cold/CBF1 enhances the accumulation of a green fluorescent protein (GFP)-tagged DELLA protein (GFP-RGA) by reducing GA content through stimulating expression of GA-inactivating GA 2-oxidase genes. PMID: 18757556
    15. Data documented that Lys-29 of ubiquitin is the major site for ubiquitin chain formation to mediate DELLA protein degradation that also required protein Ser/Thr dephosphorylation activity. The LZ domain is essential for both their stability and activity. PMID: 19717618

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    GRAS family, DELLA subfamily
  • 组织特异性：
    Ubiquitously expressed. Expressed in roots, rosette leaves, bolting and mature stems, young and mature siliques, flower buds and influorescences.
  • 数据库链接：

    KEGG: ath:AT2G01570

    STRING: 3702.AT2G01570.1

    UniGene: At.24012

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