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  4. Recombinant Arabidopsis thaliana Cytochrome P450 90B1 (CYP90B1), partial

Recombinant Arabidopsis thaliana Cytochrome P450 90B1 (CYP90B1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 90B1(CYP90B1) ,partial
  • 货号：
    CSB-YP527715DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 90B1(CYP90B1) ,partial
  • 货号：
    CSB-EP527715DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 90B1(CYP90B1) ,partial
  • 货号：
    CSB-EP527715DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 90B1(CYP90B1) ,partial
  • 货号：
    CSB-BP527715DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 90B1(CYP90B1) ,partial
  • 货号：
    CSB-MP527715DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CYP90B1
  • Uniprot No.：
    O64989
  • 别名：
    CYP90B1; DWF4; At3g50660; T3A5.40Cytochrome P450 90B1; EC 1.14.-.-; Protein DWARF 4; Dwarf4; Steroid 22-alpha-hydroxylase
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the C22-alpha-hydroxylation step in brassinosteroids biosynthesis. Converts campestanol to 6-deoxocathasterone and 6-oxocampestanol to cathasterone.
  • 基因功能参考文献：
    1. TCP1 Modulates DWF4 Expression via Directly Interacting with the GGNCCC Motifs in the Promoter Region of DWF4 in Arabidopsis thaliana PMID: 26233893
    2. Overexpression of CYP724A1 in null dwf4-102 plants functionally complements their C-22 hydroxylase deficiency. PMID: 22196800
    3. TCP1 positively regulates BR biosynthesis via its direct regulation of the transcription of DWF4. PMID: 21822059
    4. DWF4 transcription plays a novel role in the brassinosteroid (BR)-auxin crosstalk associated with root elongation, in addition to its role in BR homeostasis. PMID: 21909364
    5. The binding of BZR1 (brassinazole-resistant 1 protein) to the DWF4 promoter is attenuated by treatment with auxin. PMID: 21284753
    6. Studies indicate that DWF4 gene encodes a 22-a hydroxylase (CYP90B1) that catalyzes the conversion of 6- Oxocampestanol/Campestanol to 6-Deoxocathasterone/Cathasterone. PMID: 21103336
    7. TCP1 positively regulates the expression of the key brassinosteroid biosynthetic gene, DWF4. PMID: 20435901
    8. Brassinosteroid negatively regulates jasmonate inhibition of root growth, and DWF4 is down-regulated by jasmonate. PMID: 19741050
    9. It is likely that the DWF4 promoter serves as a focal point in maintaining homeostasis of endogenous bioactive brassinosteroids pools in specific tissues of Arabidopsis. PMID: 16407451
    10. may explain the discrepancy between the in planta abundance of C27/C28/C29 sterols and C27/C28/C29 brassinosteroids PMID: 16460510
    11. Transcripts for dwf4 accumulated in the epidermis of young leaf primordia and targeted expression of DWF4 to a subset of these cells led to both restoration of differentiation of a specific group of leaf cells and restoration of wild-type leaf shape. PMID: 17908154

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  • 亚细胞定位：
    Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Expressed in stems, leaves, shoots, and roots, with a higher expression in siliques and apical shoots.
  • 数据库链接：

    KEGG: ath:AT3G50660

    STRING: 3702.AT3G50660.1

    UniGene: At.26401

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