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  4. Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1 (COI1), partial

Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1 (COI1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1(COI1) ,partial
  • 货号：
    CSB-YP517316DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1(COI1) ,partial
  • 货号：
    CSB-EP517316DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1(COI1) ,partial
  • 货号：
    CSB-EP517316DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1(COI1) ,partial
  • 货号：
    CSB-BP517316DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1(COI1) ,partial
  • 货号：
    CSB-MP517316DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    COI1
  • Uniprot No.：
    O04197
  • 别名：
    COI1; FBL2; At2g39940; T28M21.10Coronatine-insensitive protein 1; COI-1; F-box/LRR-repeat protein 2; AtCOI1; AtFBL2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Seems to not be required for meiosis. Required for the regulation of some genes induced by wounding, but not for all. Component of SCF(COI1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including the ribulose bisphosphate carboxylase small chain 1B RBCS-1B and the histone deacetylase HDA6). These SCF complexes play crucial roles in regulating response to jasmonate, and their interactions with the COP9 signalosome (CSN) appear to be important for their activity. Interacts with TIFY10A and inositol pentakisphosphate to form a high-affinity jasmonates coreceptor. Involved in the regulation of plant gene expression during plant-pathogen interactions with Pseudomonas syringae and Alternaria brassicicola.
  • 基因功能参考文献：
    1. The CORONATINE INSENSITIVE1 (COI1)-dependent signaling pathway delays the flowering time of Arabidopsis thaliana by inhibiting the expression of the florigen gene FLOWERING LOCUS T (FT). PMID: 26410299
    2. type III effectors AvrRpm1 and AvrRpt2 promote bacterial virulence by targeting a COI1-dependent signaling pathway. PMID: 26795143
    3. Data show that the expression of CORONATINE INSENSITIVE1 (COI1)-YFP in the epidermis of the stamen filament and anther in coi1 mutant plants is sufficient to rescue filament elongation, anther dehiscence and pollen viability. PMID: 26833563
    4. Physiological and genetic evidence showed that the increased level of anthocyanin in thf1 is dependent on coronatine-insensitive1 (COI1) signaling PMID: 24467527
    5. DAD1 and DGL required COI1 for their expression, suggesting that a molecule other than jasmonic acid (JA) might act as a regulator of COI1. PMID: 24430866
    6. Jasmonoyl-isoleucine levels do not protect Arabidopsis against the fungus in the absence of COI1. PMID: 24300304
    7. The stabilization and degradation of COI1, is reported. PMID: 23733061
    8. COI1 protein is strictly regulated by a dynamic balance of SCF complex mediated stabilization and 26S proteasome-mediated degradation and thus maintained at a protein level essential for proper biological functions. PMID: 23386265
    9. Potentiation of callose deposition in ocp3 plants, including enhanced disease resistance, also required jasmonic acid (JA) recognition though a COI1 receptor, however JA was not required for basal callose deposition following fungal infection. PMID: 21564353
    10. Meristem arrest and apical dominance appear to be evolutionarily divergent functions for COI1 that are governed in an ecotype-dependent manner. PMID: 23573263
    11. Rice COI1a and COI1b complement Arabidopsis coi1-1 mutants by overexpression of either gene in the coi1-1 mutant, resulting in restoration of jasmonate signal transduction and seed production. PMID: 23320078
    12. COR also suppresses callose deposition and promotes bacterial growth in coi1 mutant plants, indicating that COR may have multiple targets inside plant cells. PMID: 23204405
    13. COI1 mediates root growth inhibition in response to phytoprostanes. PMID: 23349138
    14. a modular function for COI1 in plant growth, defence and fertility PMID: 23383073
    15. The coi1-1 mutant was less sensitive to jasmonic acid on pericycle cell activation to induce lateral root primordia (LRP) formation and presented alterations in lateral root positioning and lateral root emergence on bends. PMID: 22658222
    16. The coi1 mutation suppressed several bik1 phenotypes, suggesting that COI1 may act as a repressor of BIK1 function. PMID: 21862710
    17. These results have unraveled a novel COI1 function in the roots that acts independently from jasmonic acid (JA)-isoleucine or any JA-isoleucine mimic. PMID: 22635114
    18. These results suggest that the Low red/far-red effect, in contrast to the salicylic acid effect, requires a functional SCFCOI1-JASMONATE ZIM-DOMAIN (JAZ) JA receptor module. PMID: 22371506
    19. Additional abscissic acid response loci by suppressor screening of the jasmonate (JA)-insensitive coronatine insensitive 1-16 (coi1-16) mutant, were identified. PMID: 22156383
    20. COI1 variants with changes in putative inositol polyphosphate coordinating residues exhibited a reduced interaction with the COI1 target, JAZ9, in yeast two-hybrid tests. PMID: 21205029
    21. Findings indicate that JAZ splice variants have reduced capacity to form stable complexes with COI1 in the presence of the bioactive stereoisomer of the hormone (3R,7S)-JA-Ile. PMID: 20525008
    22. COI1 is required for the light-dependent, jasmonic acid-independent, root growth inhibition by ethylene. PMID: 20699268
    23. identification of a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand PMID: 20927106
    24. Coronatine-insensitive 1 (COI1) mediates transcriptional responses of Arabidopsis thaliana to external potassium supply. PMID: 20339157
    25. A mutation within the coi1 gene impairs methyl jasmonate-induced stomatal closing, but not abscisic acid-induced stomatal closing. PMID: 17220365
    26. Coronatine promotes interaction between COI1 and JAZ1. Two positively charged amino acid residues in the C-terminus Jas domain are essential for coronatine-dependent COI1-JAZ interactions. PMID: 18547396
    27. COI1 was required for jasmonate induced expression of the late anthocyanin biosynthetic genes. PMID: 19596700
    28. Results demonstrate that COI1 directly binds to JA-Ile and COR and serves as a receptor for jasmonate. PMID: 19717617

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  • 数据库链接：

    KEGG: ath:AT2G39940

    STRING: 3702.AT2G39940.1

    UniGene: At.20831

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