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  4. Recombinant Arabidopsis thaliana Calcium-activated outward-rectifying potassium channel 1 (KCO1), partial

Recombinant Arabidopsis thaliana Calcium-activated outward-rectifying potassium channel 1 (KCO1), partial

  • 中文名称：
    拟南芥TPK1重组蛋白
  • 货号：
    CSB-YP814494DOA1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥TPK1重组蛋白
  • 货号：
    CSB-EP814494DOA1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥TPK1重组蛋白
  • 货号：
    CSB-EP814494DOA1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥TPK1重组蛋白
  • 货号：
    CSB-BP814494DOA1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥TPK1重组蛋白
  • 货号：
    CSB-MP814494DOA1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TPK1
  • Uniprot No.：
    Q8LBL1
  • 别名：
    TPK1; KCO1; At5g55630; MDF20.7; Two-pore potassium channel 1; AtTPK1; Calcium-activated outward-rectifying potassium channel 1; AtKCO1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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TPK1 Proteins

靶点详情

  • 功能：
    Voltage-independent, large conductance and potassium-selective tonoplast ion channel. Regulated by cytoplasmic calcium and pH. Does not mediate slow-vacuolar (SV) ionic currents, but essential to establish VK currents. Has some permeability for Rb(+) and NH(4)(+), but none for Na(+), Cs(+) or Li(+). Involved in intracellular K(+) redistribution and/or K(+) retranslocation between different tissues.
  • 基因功能参考文献：
    1. ATKCO1 can form functional potassium transport systems in E. coli PMID: 23656881
    2. TPK1 must assemble correctly to pass endoplasmic reticulum quality control. PMID: 21697507
    3. data clearly demonstrate that TPK1 (KCO1) is a voltage-independent, Ca(2+)-activated, K(+)-selective ion channel in the vacuolar membrane that does not mediate SV-type ionic currents [TPK1] PMID: 16113216
    4. The two-pore channel TPK1 gene encodes the vacuolar K+ conductance and plays a role in K+ homeostasis. PMID: 17563365
    5. findings show that AtTPK1 (KCO1), interacts with 14-3-3 proteins (general regulating factors, GRFs; TPK1 seems to provide for a Ca(2+)- and 14-3-3-sensitive mechanism capable of controlling cytoplasmic potassium homeostasis in plants PMID: 17764516

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  • 亚细胞定位：
    Vacuole membrane; Multi-pass membrane protein. Note=Tonoplast.
  • 蛋白家族：
    Two pore domain potassium channel (TC 1.A.1.7) family
  • 组织特异性：
    Detected in mesophyll cells, guard cells and vascular tissues of the leaves. Expressed in the hilum, where the funiculus is attached during fruit maturation and in the embryo. Also expressed at a lower level in seedlings, root tips and elongation zones, a
  • 数据库链接：

    KEGG: ath:AT5G55630

    STRING: 3702.AT5G55630.1

    UniGene: At.20254

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