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  4. Recombinant Arabidopsis thaliana CHD3-type chromatin-remodeling factor PICKLE (PKL), partial

Recombinant Arabidopsis thaliana CHD3-type chromatin-remodeling factor PICKLE (PKL), partial

  • 中文名称：
    拟南芥PKL重组蛋白
  • 货号：
    CSB-YP879675DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PKL重组蛋白
  • 货号：
    CSB-EP879675DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PKL重组蛋白
  • 货号：
    CSB-EP879675DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PKL重组蛋白
  • 货号：
    CSB-BP879675DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PKL重组蛋白
  • 货号：
    CSB-MP879675DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PKL
  • Uniprot No.：
    Q9S775
  • 别名：
    PKL; CHR6; GYM; At2g25170; F13D4.130; CHD3-type chromatin-remodeling factor PICKLE; EC 3.6.4.-; Protein CHROMATIN REMODELING 6; AtCHR6; Protein GYMNOS
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Chromatin remodeling factor that represses the expression of embryonic trait genes (such as NFYB9/LEC1) upon and after seed germination and thus enables the developmental switch to post-germinative growth. Silences some MADS-box proteins such as PHE1 and PHE2. Plays a role during carpel differentiation. Regulates late processes in cytokinin signaling.
  • 基因功能参考文献：
    1. The antagonistic functions of FIS1 and PKR2 in modulating endosperm development are similar to those of PICKLE (PKL) and CURLY LEAF (CLF), which antagonistically regulate root meristem activity. PMID: 28155248
    2. Pickle role in the vegetative growth and gibberellin signaling PMID: 28057895
    3. Plants lacking the CHD3 remodeler, PICKLE, exhibit various reproductive defects. These phenotypes result from loss of PICKLE in the maternal sporophyte. PMID: 27075727
    4. data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants PMID: 27056257
    5. EPP1 acts additively with SPA1 to repress seedling de-etiolation. PMID: 23733056
    6. PKL physically interacts with HY5 to directly regulate hypocotyl cell elongation by repressing trimethylation of histone H3 Lys 27 (H3K27me3) on the regulatory regions of several cell elongation- related genes in response to changing light conditions. PMID: 23314848
    7. These studies demonstrate that subfamily II CHD proteins in plants, such as PICKLE, retain ATP-dependent chromatin remodeling activity PMID: 23128324
    8. CKH1/EER4/AtTAF12b and CKH2/PKL may act together on cytokinin-regulated genes. PMID: 21357580
    9. PKL plays a role in chromatin homeostasis. PMID: 22452853
    10. Loss of PKL function impairs root growth in Arabidopsis. PMID: 21441433
    11. PKL is required continuously after germination to repress expression of PHERES1, a type I MADS box gene that is normally expressed during early embryogenesis in wild-type plants. PMID: 16359393
    12. PKL-dependent repression of embryonic gene expression extends to late-embryogenesis genes and is associated with changes in chromatin. PMID: 17892443
    13. PKL promotes hisone h3 methylation in both germinating seedlings and in adult plants but do not identify a connection between PKL-dependent expression and acetylation levels. PMID: 18539592
    14. PKL and PKR2 are required for the expression of many genes that are repressed by PcG proteins. PMID: 19680533

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 组织特异性：
    Mostly expressed in tissue undergoing significant differentiation (meristems and primordia) such as young seedlings, influorescent tissue and young siliques, but not in endosperm and seed coat (at protein level). Levels decrease as organs age. Also presen
  • 数据库链接：

    KEGG: ath:AT2G25170

    STRING: 3702.AT2G25170.1

    UniGene: At.11745

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