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  4. Recombinant Arabidopsis thaliana B3 domain-containing transcription repressor VAL1 (VAL1), partial

Recombinant Arabidopsis thaliana B3 domain-containing transcription repressor VAL1 (VAL1), partial

  • 中文名称：
    拟南芥VAL1重组蛋白
  • 货号：
    CSB-YP851915DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥VAL1重组蛋白
  • 货号：
    CSB-EP851915DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥VAL1重组蛋白
  • 货号：
    CSB-EP851915DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥VAL1重组蛋白
  • 货号：
    CSB-BP851915DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥VAL1重组蛋白
  • 货号：
    CSB-MP851915DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VAL1
  • Uniprot No.：
    Q8W4L5
  • 别名：
    VAL1; HSI2; At2g30470; T6B20.17; B3 domain-containing transcription repressor VAL1; Protein HIGH-LEVEL EXPRESSION OF SUGAR-INDUCIBLE 2; Protein VP1/ABI3-LIKE 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor of gene expression involved in embryonic pathways, such as LEC1, ABI3, and FUS3. Repressor of the sugar-inducible genes involved in the seed maturation program in seedlings. Plays an essential role in regulating the transition from seed maturation to seedling growth. Functionally redundant with VAL2/HSL1.
  • 基因功能参考文献：
    1. VAL1 crystal structure provides insight into the important implication of VAL1-B3 domain in flowering process. PMID: 29733847
    2. The results suggest that HSI2 recruits MED13 and HDA6 to suppress directly a subset of seed maturation genes post-germination. PMID: 27335347
    3. LEC1, LEC2 , and FUSCA3 transcripts are candidate targets of VAL1, acting through epigenetic and/or transcriptional repression. PMID: 26678037
    4. VAL1 localizes to the nucleation region in vivo, promoting histone deacetylation and FLOWERING LOCUS C transcriptional silencing, and interacts with components of the conserved apoptosis- and splicing-associated protein complex. PMID: 27471304
    5. HSI2 PHD-like domain in the regulation of gene expression involving histone modifications and DNA methylation-mediated epigenetic mechanisms. PMID: 25367506
    6. These results implicate HSI2 as a negative regulator of drought stress response in Arabidopsis. PMID: 24168327
    7. A novel HSI2 mutant allele, hsi2-4, was identified that exhibits constitutively elevated luciferase expression while expression of the endogenous GSTF8 transcript remains unchanged. Microarray analysis indicated that the HSI2 PHD-like domain functions non-redundantly to repress a subset of seed maturation genes. [HSI2] PMID: 22476218
    8. These results indicate that HSI2 and related proteins are B3 domain-EAR motif active transcription repressors. [HSI2] PMID: 15894743

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in flowers and at lower levels in roots, stems and leaves.
  • 数据库链接：

    KEGG: ath:AT2G30470

    STRING: 3702.AT2G30470.1

    UniGene: At.27438

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