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  4. Recombinant Arabidopsis thaliana Auxin efflux carrier component 4 (PIN4), partial

Recombinant Arabidopsis thaliana Auxin efflux carrier component 4 (PIN4), partial

  • 中文名称：
    拟南芥PIN4重组蛋白
  • 货号：
    CSB-YP844580DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PIN4重组蛋白
  • 货号：
    CSB-EP844580DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PIN4重组蛋白
  • 货号：
    CSB-EP844580DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PIN4重组蛋白
  • 货号：
    CSB-BP844580DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PIN4重组蛋白
  • 货号：
    CSB-MP844580DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIN4
  • Uniprot No.：
    Q8RWZ6
  • 别名：
    PIN4; At2g01420; F2I9.4; Auxin efflux carrier component 4; AtPIN4
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a component of the auxin efflux carrier. Plays a role in generating a sink for auxin into columella cells. Maintains the endogenous auxin gradient, which is essential for correct root patterning. Involved in EXO70A3-regulated gravitropic responses in columella cells and in root system architecture (RSA).
  • 基因功能参考文献：
    1. ABA treatment reduced the expression levels of the PIN-FORMEDs (PIN) auxin efflux carriers, PIN1, PIN3, PIN4, and PIN7, to a greater extent in the root meristems of arf2-101 mutant than in the wild type. ARF2 positively mediates the transcripts of transcription factor PLETHORA 1 (PLT1) gene but negatively mediates PLT2 at protein level in root meristems. PMID: 28074634
    2. Functional interplay between protein kinase CK2 and salicylic acid sustains PIN4 transcriptional expression. PMID: 24547808
    3. Data show that phototropic hypocotyl bending is strongly dependent on the activity of D6PKs and the PIN-FORMED (PIN) auxin efflux facilitators PIN3, PIN4, and PIN7. PMID: 23709629
    4. NOV is required for cell type-specific expression of PIN4 in the root. PMID: 19880797
    5. Data suggest that auxin transport activity, in particular that of the PIN-FORMED1 (PIN1) and PIN4 proteins, is a major factor in the maintenance of auxin gradients. PMID: 16055631
    6. PP2A and PINOID both partially colocalize with PINs and act antagonistically on the phosphorylation state of their central hydrophilic loop, hence mediating PIN apical-basal polar targeting. PMID: 17889649

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Auxin efflux carrier (TC 2.A.69.1) family
  • 组织特异性：
    Expressed in the quiescent center precursors and surrounding cells. Present in columella cells of primary roots. Detected in pollen.
  • 数据库链接：

    KEGG: ath:AT2G01420

    STRING: 3702.AT2G01420.2

    UniGene: At.24881

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