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  4. Recombinant Arabidopsis thaliana 7-hydroxymethyl chlorophyll a reductase, chloroplastic (HCAR)

Recombinant Arabidopsis thaliana 7-hydroxymethyl chlorophyll a reductase, chloroplastic (HCAR)

  • 中文名称：
    拟南芥HCAR重组蛋白
  • 货号：
    CSB-YP812734DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥HCAR重组蛋白
  • 货号：
    CSB-EP812734DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥HCAR重组蛋白
  • 货号：
    CSB-EP812734DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥HCAR重组蛋白
  • 货号：
    CSB-BP812734DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥HCAR重组蛋白
  • 货号：
    CSB-MP812734DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HCAR
  • Uniprot No.：
    Q8GS60
  • 别名：
    HCAR; HMCR; At1g04620; T1G11.13; 7-hydroxymethyl chlorophyll a reductase; chloroplastic; EC 1.17.7.2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    21-462
  • 氨基酸序列
    SSSSRSKDMN LEPKKKVKLR EDWREKSRPI PPGGTYPAKD HCSQCGLCDT YYIAHVKEAC AFLGDGMSRI ESLEPVVHGR GRKADSLQDT YFGVHQEQLY ARKLKPVEGA QWTGIVTTIA IEMLKSNMVE AVVCVQSDPE DRLSPRPVLA RTPEEVLAAR GVKPTLSPNL NTLELIEASG VKRLLFCGVG CQVQALRSVE QHLNLEKLYV LGTNCVDNGT RDGLDKFLKA ASKEPETVLH YEFMQDYKVQ LKHLDGHIEE VPYFSLPAND LVDVIAPSCY SCFDYTNALA DLVIGYMGVP KYSGLNMTDH PQYITVRNER GKEMLSLVEN LLEITPTISS GDRRPFVTET VKADDAAKFG QGPAQPAPLF VGNIIAFILN LVGPKGLEFA RYSLDYHTIR NYLYVNRKWG KQRANTHMPS YAKKIVEMYN KNGQIDKMLS KK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable iron-sulfur flavoprotein that converts 7-hydroxymethyl chlorophyll a to chlorophyll a using ferredoxin as a reducing equivalent. Catalyzes the reduction of a hydroxymethyl group to a methyl group. Belongs to the chlorophyll catabolic enzymes (CCEs).
  • 基因功能参考文献：
    1. addition to functioning in the conversion of 7-HMC a to Chl a in senescent leaves, HCAR may play a critical role in protecting plants from high light-induced damage by preventing the accumulation of 7-HMC a and Pheo a in developing and mature leaves at the vegetative stage PMID: 29047257
    2. report the crystal structure of Arabidopsis HCAR at 2.7-A resolution and reveal that two [4Fe-4S]clusters and one FAD within a very short distance form a consecutive electron pathway to the substrate pocket. In vitro kinetic analysis confirms the ferredoxin-dependent electron transport chain, thus supporting a proton-activated electron transfer mechanism. PMID: 27072131
    3. in Arabidopsis, 7-hydroxymethyl chlorophyll a reductase overexpressing plants exhibited accelerated leaf yellowing and, conversely, hcar mutants stayed green during dark-induced senescence. PMID: 23200839
    4. 7-hydroxymethyl chlorophyll a reductase contains flavin adenine dinucleotide and an iron-sulfur center. It converts 7-hydroxymethyl chlorophyll a to chlorophyll a using ferredoxin. PMID: 21934147
  • 亚细胞定位：
    Plastid, chloroplast.
  • 蛋白家族：
    FrhB family
  • 数据库链接：

    KEGG: ath:AT1G04620

    STRING: 3702.AT1G04620.1

    UniGene: At.42441

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