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  4. Recombinant Arabidopsis thaliana 5'-3' exoribonuclease 4 (XRN4), partial

Recombinant Arabidopsis thaliana 5'-3' exoribonuclease 4 (XRN4), partial

  • 中文名称：
    拟南芥XRN4重组蛋白
  • 货号：
    CSB-YP880817DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥XRN4重组蛋白
  • 货号：
    CSB-EP880817DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥XRN4重组蛋白
  • 货号：
    CSB-EP880817DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥XRN4重组蛋白
  • 货号：
    CSB-BP880817DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥XRN4重组蛋白
  • 货号：
    CSB-MP880817DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    XRN4
  • Uniprot No.：
    Q9FQ04
  • 别名：
    XRN4; AIN1; EIN5; At1g54490; F20D21.305'-3' exoribonuclease 4; EC 3.1.13.-; Protein ACC INSENSITIVE 1; Protein ETHYLENE INSENSITIVE 5; Protein EXORIBONUCLEASE 4
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Possesses 5'->3' exoribonuclease activity. Acts as an endogenous post-transcriptional gene silencing (PTGS) suppressor. Degrades miRNA target cleavage products that lack a 5'-cap structure. Antagonizes the negative feedback regulation on EIN3 by promoting EBF1 and EBF2 mRNA decay, which consequently allows the accumulation of EIN3 protein to trigger the ethylene response.
  • 基因功能参考文献：
    1. These results demonstrate that AtXRN4-mediated mRNA degradation is linked to the suppression of heat acclimation. PMID: 26136597
    2. XRN4 is required to set up a massive heat-induced mRNA decay. PMID: 24332370
    3. Loss of XRN4 function can potentially trigger the cosuppression in a sequence-dependent manner. PMID: 22611176
    4. Map based cloning identified in both lines mutations in XRN4 (Exoribonuclease 4), which was previously described as a suppressor of transgene silencing in source leaves. PMID: 21039560
    5. The identification of XRN4 as an integral new component in ethylene signaling adds RNA degradation as another posttranscriptional process that modulates the perception of this plant hormone. PMID: 17085683
    6. Xrn1p is involved in TBSV RNA degradation affecting recombination. PMID: 17688902

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    5'-3' exonuclease family, XRN2/RAT1 subfamily
  • 组织特异性：
    Expressed in roots, leaves, stems and flowers.
  • 数据库链接：

    KEGG: ath:AT1G54490

    STRING: 3702.AT1G54490.1

    UniGene: At.16935

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