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  4. Recombinant Arabidopsis thaliana 4-substituted benzoates-glutamate ligase GH3.12 (GH3.12), partial

Recombinant Arabidopsis thaliana 4-substituted benzoates-glutamate ligase GH3.12 (GH3.12), partial

  • 中文名称：
    拟南芥GH3.12重组蛋白
  • 货号：
    CSB-YP878640DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥GH3.12重组蛋白
  • 货号：
    CSB-EP878640DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥GH3.12重组蛋白
  • 货号：
    CSB-EP878640DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥GH3.12重组蛋白
  • 货号：
    CSB-BP878640DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥GH3.12重组蛋白
  • 货号：
    CSB-MP878640DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GH3.12
  • Uniprot No.：
    Q9LYU4
  • 别名：
    GH3.12; GDG1; PBS3; WIN3; At5g13320; T22N19.5; T31B5.1404-substituted benzoates-glutamate ligase GH3.12; EC 6.3.2.-; Auxin-responsive GH3-like protein 12; AtGH3-12; Protein GH3-LIKE DEFENSE GENE 1; Protein GRETCHEN HAGEN 3.12; Protein HOPW1-1-INTERACTING 3; Protein avrPPHB SUSCEPTIBLE 3
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).
  • 基因功能参考文献：
    1. The X-ray crystal structure of GH3.12 is presented in the closed conformation in complex with the nonhydrolysable ATP analogue AMPCPP and the substrate salicylate. PMID: 24100325
    2. WIN3 represents a novel node in the salicylic acid signaling networks to regulate plant defense and flowering time. [WIN3] PMID: 21543726
    3. Amino acid conjugation plays a critical role in salicylic acid (SA) metabolism and induced defense responses, with PBS3 acting upstream of SA, directly on SA, or on a competitive inhibitor of SA. PMID: 17468220
    4. Molecular characterization of the GH3-LIKE DEFENSE GENE 1 (GDG1), a member of the GH3-like gene family, shows that GDG1 is an important component of salicylic acid mediated defense against the bacterial pathogen P. syringae.[GDG1] PMID: 17521413
    5. WIN3 (At5g13320) is a key element of the RPS2 defense response pathway and a basal and systemic defense component. PMID: 17918621
    6. WIN3 is partially required for Pseudomonas syringae HopW1-1-induced disease resistance. PMID: 18266921
    7. Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate PMID: 19189963

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  • 蛋白家族：
    IAA-amido conjugating enzyme family
  • 组织特异性：
    Expressed in seedlings, mostly in cotyledons, leaves, hypocotyls and sporadically in roots. Not detected in unchallenged adult plants, except in flowers.
  • 数据库链接：

    KEGG: ath:AT5G13320

    STRING: 3702.AT5G13320.1

    UniGene: At.32084

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