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  4. Recombinant Arabidopsis thaliana Arabidopsis thaliana (AUR1)

Recombinant Arabidopsis thaliana Arabidopsis thaliana (AUR1)

  • 中文名称：
    Recombinant Arabidopsis thaliana Arabidopsis thaliana(AUR1)
  • 货号：
    CSB-EP878654DOA
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    AUR1
  • Uniprot No.：
    Q9M077
  • 别名：
    AUR1; At4g32830; T16I18.40Serine/threonine-protein kinase Aurora-1; AtAur1; EC 2.7.11.1; Aurora-like kinase 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    54.0kDa
  • 表达区域：
    1-294aa
  • 氨基酸序列
    MAIPTETQHQEKEASDASAAAAQKRWTLSDFDIGKPLGRGKFGHVYLAREKRSNHVVALKVLFKSQLQQSQVEHQLRREVEIQSHLRHPNILRLYGYFYDQKRVYLILEYAARGELYKDLQKCKYFSERRAATYVASLARALIYCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMVESVEHDASVDIWSLGILCYEFLYGVPPFEAMEHSDTYRRIVQVDLKFPPKPIISASAKDLISQMLVKESSQRLPLHKLLEHPWIVQNADPSGIYRV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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AUR1 Proteins

靶点详情

  • 功能：
    Phosphorylates specifically 'Ser-10' of histone H3 in vitro and colocalizes with phosphorylated histone H3 during mitosis. Associates with cytoskeletal structures that are necessary for cytokinesis and with the microtubule spindle. Colocalizes also with gamma-tubulin and function in microtubule organizing centers (MTOCs). In contrast with the mammalian B-type Aurora, AUR1 has no kinase activity toward 'Ser-28' of histone H3.
  • 基因功能参考文献：
    1. a central role for AUR1 kinase in regulating formative division plane orientation throughout development PMID: 22045917
    2. AtAurora1 kinase may function to phosphorylate substrates that are critical to the spatiotemporal regulation of acentrosomal microtubule formation and organization. PMID: 22150830
    3. Aurora kinase AtAUR1 localized at the nuclear membrane in interphase and located in mitotic spindles and cell plates during cell division. PMID: 16028112
    4. AtAurora1 in vivo kinase activity was inhibited by hesperadin which also inhibited histone H3S10 phosphorylation. PMID: 19582900
  • 亚细胞定位：
    Nucleus membrane. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, phragmoplast. Note=Nuclear membrane in interphase cells, spindle poles at prophase, mitotic spindle from metaphase to telophase and equatorial cell plate at telophase.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
  • 组织特异性：
    Abundant in roots, flowers and flower buds, low or absent in expanded leaves, stems and siliques.
  • 数据库链接：

    KEGG: ath:AT4G32830

    STRING: 3702.AT4G32830.1

    UniGene: At.24046

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