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  4. Recombinant Apis mellifera Vitellogenin (Vg), partial

Recombinant Apis mellifera Vitellogenin (Vg), partial

In Stock
  • 货号：
    CSB-EP768142DNK
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Vg
  • Uniprot No.：
    Q868N5
  • 种属：
    Apis mellifera (Honeybee)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    32.5 kDa
  • 表达区域：
    1439-1672aa
  • 氨基酸序列
    EDETSCMLDKTRAQTFDGKDYPLRLGPCWHAVMTTYPRINPDNHNEKLHIPKDKSVSVLSRENEAGQKEVKVLLGSDKIKFVPGTTSQPEVFVNGEKIVVSRNKAYQKVEENEIIFEIYKMGDRFIGLTSDKFDVSLALDGERVMLKASEDYRYSVRGLCGNFDHDSTNDFVGPKNCLFRKPEHFVASYALISNQCEGDSLNVAKSLQDHDCIRQERTQQRNVISDSESGRLDT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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Vg Proteins

靶点详情

  • 功能：
    Precursor of the egg-yolk proteins that are sources of nutrients during embryonic development. Involved in the differentiation of honeybee larvae into queens.
  • 基因功能参考文献：
    1. A tight coregulatory relationship exists between Vg and juvenile hormone (JH) in the regulation of honeybee behavioral maturation that is manifest at the genomic level; acting through common pathways, they regulate brain gene expression and behavior. PMID: 23889463
    2. The study demonstrates a causal link between the Vg knockdown forager phenotype and variation in the abundance of microRNAs in different tissues, with possible consequences for the regulation of foraging behavior. PMID: 23788711
    3. spectroscopic results strengthen our model of the Vg polyserine tract as a flexible domain linker shielded by phosphorylation PMID: 22573762
    4. identify vitellogenin protein in the hypopharyngeal glands (brood food producing head glands) of nursing worker bees and in adjacent head fat body cells for the first time PMID: 20337562
    5. Data show that silencing of vitellogenin expression causes a significant increase in juvenile hormone titer and its putative receptor. PMID: 16122739
    6. Vitellogenin protein, which is synthesized at high levels in honey bee queens and is abundant in long-lived workers, protects worker bees from oxidative stress PMID: 16418279
    7. In precocious foragers, downregulation of vitellogenin gene activity apparently has little effect on the juvenile hormone titer. PMID: 18545981
    8. Workers in the queenless colonies with active ovaries that have an upregulation of vit transcript expression levels, and thus assumed vitellogenin protein levels. PMID: 19096928
    9. Bacteria- or water-injection induce an equivalent and strong depression in the levels of Vg protein, which was corroborated in M. luteus-injected bees, but not in S. marcescens-injected ones, by a statistically significant decrease in vg mRNA levels. PMID: 19309002

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Accumulates in the hemolymph. Represents up to 70% of the queen's hemolymph proteins. During the first week of the worker adult life, when it becomes a nurse bee and performs brood-rearing tasks, the vitellogenin titer increases and may account for up to
  • 数据库链接：

    KEGG: ame:406088

    UniGene: Ame.206

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