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  4. Recombinant ATP-dependent RNA helicase glh-1 (glh-1), partial

Recombinant ATP-dependent RNA helicase glh-1 (glh-1), partial

  • 中文名称：
    秀丽隐杆线虫glh-1重组蛋白
  • 货号：
    CSB-YP327356CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫glh-1重组蛋白
  • 货号：
    CSB-EP327356CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫glh-1重组蛋白
  • 货号：
    CSB-EP327356CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫glh-1重组蛋白
  • 货号：
    CSB-BP327356CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫glh-1重组蛋白
  • 货号：
    CSB-MP327356CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    glh-1
  • Uniprot No.：
    P34689
  • 别名：
    glh-1; T21G5.3ATP-dependent RNA helicase glh-1; EC 3.6.4.13; Germline helicase 1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable ATP-binding RNA helicase. May act redundantly with the P-granule component glh-4 to regulate the formation of the granular structure of P-granules in embryos. Plays a role in positively regulating the localization of pgl-1 to P-granules. May play a role in transgenerational epigenetic inheritance. May protect somatic cells from excessive apoptosis during normal development.
  • 基因功能参考文献：
    1. we show that removal of either of two critical P-granule components, PGL-1 or GLH-1, is sufficient to cause germ cells to express UNC-119::GFP and MYO-3 and to display RNA accumulation defects similar to those observed after depletion of P granules. Our data identify P granules as critical modulators of the germline transcriptome and guardians of germ cell fate. PMID: 28258184
    2. Data show that GLH-1 and its FG domain are not sufficient to form granules, but require factors like PGL-1 to nucleate the localized concentration of GLH proteins. PMID: 21402789
    3. the GLH-1/DCR-1 complex may function in the transport, deposition, or regulation of maternally-transcribed mRNAs and their associated miRNAs. PMID: 21146518
    4. KGB-1 and CSN-5 regulate GLH-1 levels, with GLH-1 targeted for proteosomal degradation by KGB-1 and stabilized by CSN-5. PMID: 17699606
  • 亚细胞定位：
    Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region.
  • 蛋白家族：
    DEAD box helicase family, DDX4/VASA subfamily
  • 数据库链接：

    KEGG: cel:CELE_T21G5.3

    STRING: 6239.T21G5.3

    UniGene: Cel.19653

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