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TOMM22 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    TOMM22重组抗体
  • 货号：
    CSB-RA277640A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
      All lanes: TOMM22 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 16 kDa
      Observed band size: 18 kDa
    • IHC image of CSB-RA277640A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA277640A0HU TOMM22重组单克隆抗体是针对线粒体外膜转位酶复合体22（TOMM22）靶点开发的高特异性科研工具。该抗体识别的TOMM22蛋白是线粒体外膜转位酶（TOM）复合物的核心组分，在介导核编码蛋白跨线粒体膜运输过程中起关键作用，其功能异常与细胞能量代谢失调及程序性死亡调控密切相关。经严格验证，本产品在Western Blot实验中可检测到单一清晰目标条带，免疫组化实验显示其在线粒体膜结构的特异性定位，推荐使用稀释度为WB 1:500-1:5000、IHC 1:50-1:200，同时在ELISA平台中展现优异抗原结合能力。适用于研究线粒体蛋白质转运机制、细胞能量代谢动态平衡及凋亡信号通路调控等基础生命科学领域，可为线粒体相关疾病模型的分子机制探索、药物靶点筛选提供可靠检测支持。该重组单克隆抗体具有批次稳定性高、交叉反应性低的特点，满足细胞生物学、分子病理学等研究中对线粒体功能检测的技术需求。
  • Uniprot No.：
    Q9NS69
  • 基因名：
    TOMM22
  • 别名：
    Mitochondrial import receptor subunit TOM22 homolog (hTom22) (1C9-2) (Translocase of outer membrane 22 kDa subunit homolog), TOMM22, TOM22
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human TOMM22
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7C11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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TOMM22 antibodies

TOMM22 Proteins

靶点详情

  • 功能：
    Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
  • 基因功能参考文献：
    1. A directed proteomic approach discovered the novel interaction of BKCa with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). PMID: 27592226
    2. Tom22 is essential for metabolic conversion, as its knockdown by small interfering RNA (siRNA) completely ablated progesterone conversion in both steroidogenic mouse Leydig MA-10 and human adrenal NCI cells. PMID: 26787839
    3. Blockade of mitochondrial protein import triggers the recruitment of PARK2, by PINK1, to the TOMM machinery. PMID: 24149440
    4. Studies indicate that PUMA and TOMM22 as potential targets for miRNA-BART viral transcripts. PMID: 22178394
    5. The interaction with the cytosolic domain of Tom22 helps Bax to acquire a conformation able to interact with the outer mitochondrial membrane. PMID: 22198199
    6. the cytosolic domains of Tom20 and Tom22 function to maintain their substrate preproteins unfolded and prevent them from aggregating on the mitochondrial surface PMID: 14699115
    7. We have identified TOM22, a component of the translocase of the outer mitochondrial membrane (TOM), as a mitochondrial receptor of Bax. PMID: 17096026

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass membrane protein.
  • 蛋白家族：
    Tom22 family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 18002

    OMIM: 607046

    KEGG: hsa:56993

    STRING: 9606.ENSP00000216034

    UniGene: Hs.595072

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