SAE1 Recombinant Monoclonal Antibody

中文名称：

SAE1重组抗体
货号：

CSB-RA987582A0HU
规格：

￥1320
图片：
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: SAE1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 30, 34 kDa
Observed band size: 39 kDa

Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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产品描述：

CSB-RA987582A0HU SAE1重组单克隆抗体是针对SUMO激活酶复合物关键亚基SAE1研发的高特异性检测工具。该抗体靶向的SAE1蛋白参与调控SUMO化修饰过程，这一翻译后修饰机制在细胞周期进程、DNA损伤修复及蛋白定位等基础生物学过程中发挥重要作用。经多种技术平台验证，本产品在Western Blot（WB）和免疫荧光（IF）实验中展现出优异性能，推荐使用浓度分别为1:500-1:5000和1:20-1:200，其高亲和力可清晰识别内源性SAE1蛋白表达。在ELISA体系中同样具备稳定检测能力，适用于多种实验体系的需求。科研人员可利用该抗体深入探索SUMO化修饰通路在肿瘤发生、神经退行性疾病等病理生理模型中的作用机制，为蛋白质翻译后修饰研究、细胞应激反应分析及药物靶点筛选提供可靠工具。产品严格通过质控验证，确保批次间稳定性，满足细胞生物学、分子机制研究等领域的实验需求。
Uniprot No.：

Q9UBE0
基因名：

SAE1
别名：

SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A
反应种属：

Human
免疫原：

A synthesized peptide derived from human SAE1
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

9D8
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:5000

IF 1:20-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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功能：

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
基因功能参考文献：
1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype. PMID: 26403403
2. as it has been shown by flow cytometry analysis, specific knockdown of SAE1 slowed down the cell population at G0/G1 phase and induced apoptosis of RKO cells. PMID: 25842831
3. SUMO-1 controls the protein stability and the biological function of phosducin. PMID: 16421094
亚细胞定位：

Nucleus.
蛋白家族：

Ubiquitin-activating E1 family
组织特异性：

Expression level increases during S phase and drops in G2 phase (at protein level).
数据库链接：

HGNC: 30660

OMIM: 613294

KEGG: hsa:10055

STRING: 9606.ENSP00000270225

UniGene: Hs.515500

SAE1 Recombinant Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200