Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody

中文名称：

磷酸化-PAK4/PAK5/PAK6 (S474/S560/S602)重组抗体
货号：

CSB-RA017408A474phHU
规格：

￥1320
图片：
Western Blot
Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64,75,80 KDa
Observed band size: 75,80 KDa

Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA017408A474phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody（CSB-RA017408A474phHU）是一款高特异性抗体，靶向p21激活激酶家族成员PAK4、PAK5和PAK6的磷酸化修饰位点S474、S560及S602。该抗体经ELISA、Western blot（WB）和免疫荧光（IF）严格验证，适用于检测内源性蛋白的磷酸化状态。在WB实验中推荐使用1:500-1:5000的稀释范围，可清晰识别目标条带；IF实验推荐1:20-1:200稀释度，能精准定位蛋白在细胞内的空间分布。PAK4/5/6作为Rho家族GTP酶下游效应分子，参与调控细胞骨架重组、迁移及生存信号通路，其磷酸化修饰与激酶活性及功能调控密切相关。本抗体可为研究细胞极性建立、肿瘤侵袭转移、神经发育等领域的分子机制提供可靠工具，适用于基础科研中细胞或组织样本的蛋白表达水平分析、亚细胞定位研究及相关信号通路的功能探索。
Uniprot No.：

O96013 Q9P286 Q9NQU5
反应种属：

Human
免疫原：

A synthesized peptide derived from Human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

2D1
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:5000

IF 1:20-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200