Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Recombinant Monoclonal Antibody

中文名称：

磷酸化-MAPK8/MAPK9/MAPK10 (T183/T183/T221)重组抗体
货号：

CSB-RA013466A183phHU
规格：

￥1320
图片：
Western Blot
Positive WB detected in 293 whole cell lysate(treated with EGF or not)
All lanes Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46,54 KDa
Observed band size: 46,54 KDa

Immunocytochemistry analysis of CSB-RA013466A183phHU diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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产品描述：

该重组单克隆抗体靶向MAPK8/JNK1、MAPK9/JNK2和MAPK10/JNK3在Thr183/Thr183/Thr221位点的磷酸化修饰，可特异性识别JNK家族活化形式。JNK信号通路在细胞应激响应、增殖调控及凋亡过程中发挥核心作用，其磷酸化激活与神经退行性疾病、肿瘤发生等病理机制密切相关。经严格验证，该抗体在Western Blot中可检测多种细胞裂解液样本，呈现单一明确条带；在免疫组化实验中能清晰显示石蜡包埋组织的亚细胞定位信号，且背景干扰低。推荐应用于体外实验中JNK信号通路的激活状态检测，如氧化应激模型构建、药物靶点筛选或基因功能研究中信号转导机制解析。采用重组抗体技术生产，确保批次间高一致性和稳定性，适用于需要定量分析的ELISA检测体系及高分辨率的免疫印迹实验，为细胞信号转导研究提供可靠工具。
Uniprot No.：

P45983 P45984 P53779
反应种属：

Human
免疫原：

A synthesized peptide derived from Human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

1A9
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IHC
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:5000

IHC 1:50-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Recombinant Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200