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  4. Phospho-Histone H1.4 (T17) Recombinant Monoclonal Antibody

Phospho-Histone H1.4 (T17) Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    磷酸化-组蛋白 H1.4 (T17)重组抗体
  • 货号：
    CSB-RA010380A17phHU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-RA010380A17phHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：
    CSB-RA010380A17phHU是一款特异性识别组蛋白H1.4第17位苏氨酸磷酸化修饰位点（T17）的高灵敏度抗体。组蛋白H1家族是染色质高级结构的关键调控因子，其磷酸化修饰参与细胞周期进程、DNA损伤修复及染色质动态重塑等生物学过程。该抗体经ELISA、免疫组织化学（IHC）和免疫荧光（IF）严格验证，在IHC应用中推荐稀释比为1:50-1:500，可清晰显示组织切片中磷酸化H1.4的核内定位；IF实验推荐使用1:30-1:200稀释度，能够特异性标记细胞核内靶标信号，适用于活细胞或固定样本的亚细胞定位分析。该产品采用重组单克隆技术生产，具有高批次一致性和低交叉反应性，可广泛应用于研究有丝分裂调控、染色质凝聚机制以及表观遗传修饰相关领域，为探索细胞周期异常疾病（如肿瘤发生）或DNA损伤应答通路提供可靠工具。
  • Uniprot No.：
    P10412
  • 基因名：
    HIST1H1E
  • 别名：
    H1 histone family member 4 antibody; H1.4 antibody; H14_HUMAN antibody; H1E antibody; H1F4 antibody; Hist1h1e antibody; Histone 1 H1e antibody; Histone cluster 1 H1e antibody; Histone H1 antibody; Histone H1.4 antibody; Histone H1B antibody; MGC116819 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3E1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:500
    IF 1:30-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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HIST1H1E antibodies

HIST1H1E Proteins

靶点详情

  • 功能：
    Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
  • 基因功能参考文献：
    1. Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. PMID: 26209608
    2. This study identified and confirmed HIST1H1E protein changes within the postsynaptic density in schizophrenia. PMID: 25048004
    3. the N-terminal domain of H1 is an important determinant of affinity and specificity of H1-chromatin interactions. PMID: 22425985
    4. PKA-mediated H1.4S35 phosphorylation dissociates H1.4 from mitotic chromatin but also suggest that this phosphorylation is necessary for specific mitotic functions. PMID: 21852232
    5. Allele-specific underacetylation of histone H4 downstream from promoter is associated with X-inactivation in human cells. PMID: 12498347
    6. the lysine residue adjacent to the phosphorylation site found on the serine residue on the H1.4 peptide KARKSAGAAKR was also shown to be methylated, raising the question of whether the hypothesized "methyl/phos" switch could be extended to linker histones PMID: 15595731
    7. Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins PMID: 19144645

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  • 相关疾病：
    Rahman syndrome (RMNS)
  • 亚细胞定位：
    Nucleus. Chromosome. Note=Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.
  • 蛋白家族：
    Histone H1/H5 family
  • 数据库链接：

    HGNC: 4718

    OMIM: 142220

    KEGG: hsa:3008

    STRING: 9606.ENSP00000307705

    UniGene: Hs.248133

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