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  4. PCYT1A Recombinant Monoclonal Antibody

PCYT1A Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    PCYT1A重组抗体
  • 货号：
    CSB-RA213218A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate
      All lanes: PCYT1A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42 kDa
      Observed band size: 42 kDa
    • Immunofluorescence staining of Hela Cells with CSB-RA213218A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：
    CSB-RA213218A0HU是针对PCYT1A靶点研发的重组单克隆抗体，经多种实验验证具有优异性能。PCYT1A（磷酸胆碱胞苷酰转移酶1A）是催化磷脂酰胆碱合成的限速酶，参与细胞膜形成、脂质代谢及信号转导等基础生物学过程。本抗体经ELISA验证可特异性识别天然及重组PCYT1A蛋白，在Western Blot实验中能清晰检测内源性PCYT1A表达，推荐使用1:500-1:5000稀释比例；免疫荧光实验显示其在细胞质中呈现特异性定位信号，推荐1:20-1:200稀释范围。该抗体采用重组表达技术制备，具有高亲和力与批次间一致性，适用于探究PCYT1A在脂代谢调控、细胞膜动态平衡及相关疾病模型中的作用机制，可广泛应用于生物化学、细胞生物学等领域的基础研究，包括但不限于肝脏脂质稳态研究、神经细胞膜功能分析以及代谢通路相互作用探索等实验场景。
  • Uniprot No.：
    P49585
  • 基因名：
    PCYT1A
  • 别名：
    Choline-phosphate cytidylyltransferase A (EC 2.7.7.15) (CCT-alpha) (CTP:phosphocholine cytidylyltransferase A) (CCT A) (CT A) (Phosphorylcholine transferase A), PCYT1A, CTPCT PCYT1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human PCYT1A
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4F2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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PCYT1A Proteins

靶点详情

  • 功能：
    Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.
  • 基因功能参考文献：
    1. There is no correlation between single PCYT1A rs712012 and PCYT1A rs7639752 polymorphisms and the incidence of intrauterine fetal death. PMID: 28509322
    2. PCYT1A mutations were identified in patients with isolated retinal dystrophy without any skeletal involvement from two Italian families. PMID: 28272537
    3. CCT contributes to phospholipid compositional homeostasis. [Review] PMID: 26165797
    4. PCYT1A-generated phosphatidylcholine has a role in the normal function of white adipose tissue and insulin action PMID: 24889630
    5. We report loss-of-function mutations in PCYT1A as the cause of spondylometaphyseal dysplasia with cone-rod dystrophy. PMID: 24387990
    6. Mutations in PCYT1A cause spondylometaphyseal dysplasia with cone-rod dystrophy PMID: 24387991
    7. N-Methylaspartate induced nitric oxide synthase activation and nuclear factor-kB subunit p65 nuclear translocation in A549 cells were responsible for decreased CTP:phosphocholine cytidylyltransferase A expression PMID: 20661636
    8. Analyses showed genotype effects of PCYT1A genes on spina bifida risk, but did not show evidence of gene-nutrient. interactions. PMID: 17184542

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  • 相关疾病：
    Spondylometaphyseal dysplasia with cone-rod dystrophy (SMDCRD)
  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Endoplasmic reticulum. Nucleus.
  • 蛋白家族：
    Cytidylyltransferase family
  • 组织特异性：
    Brain, placenta, liver, fetal and adult lung.
  • 数据库链接：

    HGNC: 8754

    OMIM: 123695

    KEGG: hsa:5130

    STRING: 9606.ENSP00000292823

    UniGene: Hs.135997

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