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  4. NDUFS3 Recombinant Monoclonal Antibody

NDUFS3 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    NDUFS3重组抗体
  • 货号：
    CSB-RA224121A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate,
      All lanes: NDUFS3 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 31, 15 KDa
      Observed band size: 31 kDa
    • IHC image of CSB-RA224121A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA224121A0HU NDUFS3重组单克隆抗体是针对线粒体复合物I核心亚基NDUFS3研发的高特异性检测工具。该靶标作为线粒体呼吸链复合物I（NADH脱氢酶）的重要组成部分，直接参与细胞氧化磷酸化过程，在能量代谢稳态维持中发挥关键作用。本抗体采用重组技术制备，经ELISA、Western blot（WB）和免疫组化（IHC）多平台验证，在WB实验中可识别天然构象的NDUFS3蛋白，推荐稀释度1:500-1:5000范围内均显示清晰单一目标条带；IHC应用在1:50-1:200稀释条件下能精准定位线粒体区域，适用于石蜡包埋组织切片的亚细胞结构分析。其高亲和力和低交叉反应性特点，使之成为研究线粒体功能调控、能量代谢异常相关疾病机制（如神经退行性疾病、代谢综合征）的重要工具，可广泛用于细胞生物学模型构建、蛋白互作研究及病理状态下的分子表达分析等基础科研领域。产品严格进行批次间质控，确保实验数据的可重复性和可靠性。
  • Uniprot No.：
    O75489
  • 基因名：
    NDUFS3
  • 别名：
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-30kD) (CI-30kD) (NADH-ubiquinone oxidoreductase 30 kDa subunit), NDUFS3
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human NDUFS3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1H12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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NDUFS3 antibodies

NDUFS3 Proteins

靶点详情

  • 功能：
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
  • 基因功能参考文献：
    1. identified a novel Leigh syndrome causing NDUFS3 mutation and expanded the clinical spectrum caused by NDUFS3 mutations in this study. PMID: 30140060
    2. The results suggest that the impaired mitochondrial activity could be due to the broken interaction between DJ-1 and NDUFS3 and that downregulation of DJ-1 in sperm and testes contributes to AS pathogenesis. PMID: 29849492
    3. The NDUFS3 expressed in the temporal cortex in patient with late-onset Alzheimer's disease. PMID: 28242297
    4. High NDUFS3 expression is associated with IgA nephropathy. PMID: 25770168
    5. pH stability of w-t and Leigh syndrome mutant varied at extreme acidic pH. the molten globule like structure of w-t at pH1 was absent in case of the mutant protein. Both the w-t and mutant proteins showed multi-step thermal and Gdn-HCl induced unfolding PMID: 24028823
    6. NDUFS3 is down-regulated in serous ovarian adenocarcinoma PMID: 23446378
    7. Expressions of GRIM-19, NDUFS3, and extracellular matrix elements are correlated with invasive capabilities of breast cancer cell lines. PMID: 23630608
    8. a novel, biomarker potential of a mitochondrial complex I subunit protein, NDUFS3 - as a robust indicator of breast cancer progression and invasiveness as well as of hypoxia/necrosis in clinical specimens of invasive ductal carcinoma, was uncovered. PMID: 21867691
    9. Decreased protein levels of complex I 30-kDa subunit in fetal Down syndrome brains. PMID: 11771736

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  • 亚细胞定位：
    Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
  • 蛋白家族：
    Complex I 30 kDa subunit family
  • 数据库链接：

    HGNC: 7710

    OMIM: 603846

    KEGG: hsa:4722

    STRING: 9606.ENSP00000263774

    UniGene: Hs.502528

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