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GABRA5 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    GABRA5重组抗体
  • 货号：
    CSB-RA559038A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Ntera-2 whole cell lysate, HEK293 whole cell lysate
      All lanes: GABA A Receptor alpha 5 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 53 kDa
      Observed band size: 70 kDa
    • IHC image of CSB-RA559038A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：
    GABRA5 Recombinant Monoclonal Antibody（CSB-RA559038A0HU）是针对γ-氨基丁酸A型受体α5亚基（GABRA5）的高特异性科研工具。GABRA5是中枢神经系统GABA-A受体的重要组成亚基，主要分布于海马和大脑皮层，通过介导抑制性神经递质GABA的氯离子通道功能参与突触可塑性调节，与认知功能及神经精神疾病密切相关。该抗体经ELISA、Western Blot（WB）和免疫组化（IHC）多平台验证，在WB实验中可精准识别天然蛋白，推荐使用稀释度为1:500-1:5000；在IHC应用中能清晰显示石蜡包埋组织样本中靶蛋白的亚细胞定位，推荐稀释度1:50-1:200。其重组单克隆特性保证了批次间的高度一致性，适用于神经递质受体表达调控研究、脑区特异性功能解析以及神经退行性疾病模型的分子机制探索。本产品为神经系统基础研究提供可靠工具，可用于蛋白互作分析、受体分布图谱构建及相关信号通路的功能研究，严格限于科研领域使用。
  • Uniprot No.：
    P31644
  • 基因名：
    GABRA5
  • 别名：
    Gamma-aminobutyric acid receptor subunit alpha-5 (GABA(A) receptor subunit alpha-5), GABRA5
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human GABA A Receptor alpha 5
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    5C11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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GABRA5 antibodies

GABRA5 Proteins

靶点详情

  • 功能：
    Ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapse.
  • 基因功能参考文献：
    1. Low GABRA5 expression typified hyperproliferative tumors, and loss of taurine signaling correlated with reduced patient survival, suggesting this tumor suppressive mechanism operates in vivo. PMID: 29787571
    2. Study found a significant reduction in [(11)C]Ro15 4513 binding in the nucleus accumbens in an opiate-dependent compared with the healthy control group. Study suggests that reduced GABA A receptor alpha5 levels in the nucleus accumbens are associated with addiction. PMID: 26876472
    3. Report benzodiazepine derivative as GABA-site inhibitor of extra-synaptic GABAA alpha5 receptors. PMID: 26169564
    4. Study found that a significant portion of GABAergic postsynaptic compartments contain the alpha5 GABAAR subunit, both in vitro and in vivo PMID: 25663431
    5. This study showed that in male groups, the expression of GABRA5 was generally lower in schizophrenia cases compared to the control. PMID: 25660468
    6. Findings provide genetic evidence for the involvement of the genes GABRB3 and GABRA5 in the susceptibility to panic disorder PMID: 24755890
    7. 1,2-dichlorohexafluorocyclobutane enhancement of GABRA5 activity is abolished by GABRB3 mutations. PMID: 25211390
    8. The alpha5 nicotinic receptor GABAergic neurons form a link from the medial habenula to the serotonergic brain centers. PMID: 24227714
    9. These results provide initial evidence of a GABAA alpha5 deficit in autism spectrum disorder and support further investigations of the GABA system in this disorder. PMID: 22546616
    10. In subjects with schizophrenia, mean GABA (A) alpha5 rerceptor subunit mRNA expression is 15% lower in layer 4 of the dorsolateral prefrontal cortex. PMID: 20843900
    11. These results suggest that the GABRA5 gene may confer susceptibility to bipolar I disorder. PMID: 15882799
    12. GABA(A) receptor alpha5 subunit as a candidate gene for autism and bipolar disorder; observations suggest parent-of-origin and gain-of-function effects PMID: 17353214

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRA5 sub-subfamily
  • 数据库链接：

    HGNC: 4079

    OMIM: 137142

    KEGG: hsa:2558

    STRING: 9606.ENSP00000335592

    UniGene: Hs.24969

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