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  4. ACO2 Recombinant Monoclonal Antibody

ACO2 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    ACO2重组抗体
  • 货号：
    CSB-RA218127A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA218127A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA218127A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA218127A0HU ACO2重组单克隆抗体是针对线粒体乌头酸酶2（ACO2）开发的高特异性检测试剂，适用于酶联免疫吸附实验（ELISA）和免疫组织化学（IHC）分析。该抗体靶向的ACO2是线粒体三羧酸循环中的关键酶，负责催化柠檬酸与异柠檬酸之间的异构化反应，在细胞能量代谢和铁硫簇组装过程中发挥重要作用。经实验验证，该抗体在IHC应用中展现出优异的组织特异性，推荐使用1:50-1:200稀释比例时能清晰显示人源样本中ACO2蛋白的亚细胞定位，其显色信号与线粒体分布特征高度吻合，背景干扰极低。在ELISA平台中可实现对ACO2蛋白的定量检测，线性范围符合常规实验需求。本产品适用于能量代谢调控机制研究、线粒体功能障碍相关疾病模型的蛋白表达分析，以及氧化应激条件下酶活性变化的关联性探索等科研领域，为解析代谢性疾病、神经退行性疾病及铁代谢异常的分子机制提供可靠工具。
  • Uniprot No.：
    Q99798
  • 基因名：
    ACO2
  • 别名：
    Aconitate hydratase, mitochondrial (Aconitase) (EC 4.2.1.3) (Citrate hydro-lyase), ACO2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Aconitase 2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    8H4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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ACO2 antibodies

ACO2 Proteins

靶点详情

  • 功能：
    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  • 基因功能参考文献：
    1. Aco2 activity correlated significantly with motor score, independence scale, and functional capacity of the Unified Huntington's Disease Rating Scale as well as disease duration. Our study provides a potential biomarker to assess the disease status of HD patients and PreHD carriers. PMID: 29160844
    2. Results suggest that ACO2 activity is reduced in peripheral lymphocytes of subjects with Alzheimer's disease and mild cognitive impairment and correlates with antioxidant protection PMID: 25322927
    3. Our study shows that autosomal recessive ACO2 mutations can cause either isolated or syndromic optic neuropathy. PMID: 25351951
    4. immunofluorescence staining localized ACO2 to the human sperm mid-piece. By immunoblotting, we demonstrated that the level of ACO2 protein in asthenozoospermic samples was significantly decreased compared with that in normal fertile men PMID: 24785945
    5. Ogg1 chaperoning of Aco-2 in preventing oxidant-mediated mtDNA damage and apoptosis may afford an innovative target for the molecular events underlying oxidant-induced toxicity. PMID: 24429287
    6. Hypoxia upregulates the gene expression of mitochondrial aconitase in prostate carcinoma cells PMID: 23709747
    7. Gastric cancer patients with lower ACO2 expression have a shorter survival time than those with higher ACO2 expression. PMID: 23550275
    8. Homozygosity mapping followed by whole-exome sequencing disclosed a Ser112Arg mutation in ACO2. PMID: 22405087
    9. these results suggest that p53 downregulation of mACON gene expression in human prostate carcinoma cells may not occur through the putative consensus p53 response elements found within the mACON promoter. PMID: 20607720
    10. abolishes oxidant-induced apoptosis PMID: 19524665
    11. Lon protease selectively recognizes and degrades the oxidized, hydrophobic form of aconitase after mild oxidative modification, but that severe oxidation results in aconitase aggregation, which makes it a poor substrate for Lon. PMID: 12198491
    12. ACO2 is often deleted in colorectal cancer but is unlikely to be the true target of the deletions PMID: 12746427
    13. The m-aconitase promoter is contained in a 153-bp 5' fragment lacking a TATA or CAAT sequence. Sp1 binding to specific Sp1 site is needed for promoter activity. Other transcription factors are recruited through protein-protein interactions. PMID: 16598741
    14. Manganese acts as an antagonist of iron, disrupting the enzymatic activity and gene expression of mACON and citrate metabolism in the prostate. PMID: 16625280
    15. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953
    16. This protein has been found differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19110265

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  • 相关疾病：
    Infantile cerebellar-retinal degeneration (ICRD); Optic atrophy 9 (OPA9)
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Aconitase/IPM isomerase family
  • 数据库链接：

    HGNC: 118

    OMIM: 100850

    KEGG: hsa:50

    STRING: 9606.ENSP00000216254

    UniGene: Hs.643610

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