malE Recombinant Monoclonal Antibody

中文名称：

malE重组抗体
货号：

CSB-RA846828A0ENV
规格：

￥1320
图片：
Western Blot
Positive WB detected in Escherichia coli lysate
All lanes: malE antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
其他：

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产品描述：

CSB-RA846828A0ENV malE重组单克隆抗体是一款经ELISA及Western Blot（WB）验证的高特异性科研工具，靶向细菌麦芽糖结合蛋白（MalE/MBP）。该抗体通过重组表达技术制备，可精准识别原核表达系统中表达的MalE蛋白，适用于检测带有MBP标签的融合蛋白及分析细菌麦芽糖转运相关机制。在验证实验中，抗体在WB中呈现单一清晰的目标条带，且ELISA检测显示线性范围宽泛、灵敏度优异，适用于低丰度样本分析。其应用场景涵盖分子生物学研究中重组蛋白纯化工艺的质控、原核表达系统蛋白表达水平的检测，以及微生物代谢途径相关蛋白的功能研究。产品经严格质检，具有批次稳定性和高重复性，可为细菌蛋白互作、原核表达体系优化等基础研究提供可靠支持。
Uniprot No.：

P0AEX9
基因名：

malE
别名：

Maltose-binding periplasmic protein (MBP) (MMBP) (Maltodextrin-binding protein), malE
反应种属：

Escherichia coli
免疫原：

A synthesized peptide derived from Escherichia coli malE
免疫原种属：

Escherichia coli (strain K12)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

10F2
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB
推荐稀释比：

Application Recommended Dilution

WB 1:1000-1:5000
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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功能：

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
基因功能参考文献：
1. Upon refolding, MBP initially collapses into a dynamic molten globule-like ensemble, then forms an obligatory on-pathway native-like folding intermediate that brings together sequentially remote segments and then folds globally after a long delay. PMID: 29295923
2. Our current understanding of what makes MBP such an effective solubility enhancer for other proteins and how it facilitates the proper folding of its fusion partners is presented [REVIEW] PMID: 28132493
3. Maltose Binding Protein is key to the coupling of maltose transport to ATP hydrolysis in vivo, because it facilitates the progression of the MalK dimer from the open to the semi-open conformation, from which it can proceed to hydrolyze ATP PMID: 26268698
4. activates mouse macrophages and polarizes them into M1 macrophages PMID: 24825603
5. a salt-bridge motif in the maltose-binding protein has a role in the initial steps of substrate transport PMID: 16143635
6. Our findings indicate that the solubility-enhancing activity of MBP is mediated by its open conformation and point to a likely role for the ligand-binding cleft in the mechanism of solubility enhancement. PMID: 17964542
7. These data indicate that unfolding of the mature domain of preMBP is likely not a rate-determining step in translocation when the protein is targeted to the translocase via SecB. PMID: 18241889
8. tat mutants are able to transport maltose in the absence of malE. PMID: 18385983
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亚细胞定位：

Periplasm.
蛋白家族：

Bacterial solute-binding protein 1 family
数据库链接：

KEGG: ecj:JW3994

STRING: 316385.ECDH10B_4223

malE Recombinant Monoclonal Antibody

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